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High-throughput strategies for the discovery and engineering of enzymes for biocatalysis

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Abstract

Innovations in novel enzyme discoveries impact upon a wide range of industries for which biocatalysis and biotransformations represent a great challenge, i.e., food industry, polymers and chemical industry. Key tools and technologies, such as bioinformatics tools to guide mutant library design, molecular biology tools to create mutants library, microfluidics/microplates, parallel miniscale bioreactors and mass spectrometry technologies to create high-throughput screening methods and experimental design tools for screening and optimization, allow to evolve the discovery, development and implementation of enzymes and whole cells in (bio)processes. These technological innovations are also accompanied by the development and implementation of clean and sustainable integrated processes to meet the growing needs of chemical, pharmaceutical, environmental and biorefinery industries. This review gives an overview of the benefits of high-throughput screening approach from the discovery and engineering of biocatalysts to cell culture for optimizing their production in integrated processes and their extraction/purification.

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Acknowledgments

The REALCAT platform benefits from a Governmental subvention administered by the French National Research Agency (ANR) within the frame of the ‘Future Investments’ program (PIA), with the contractual reference ‘ANR-11-EQPX-0037’. The Nord-Pas-de-Calais Region and the FEDER are thanked for their financial contribution to the acquisition of the equipment of the platform. The Université Lille 1 Sciences & Technologies (USTL) is also acknowledged for having financed a part of the consulting fees for designing the program and the Ecole Centrale de Lille (ECLille) is warmly thanks for their financial contribution necessary for the setup of the implantation laboratories. The consulting was entrusted to the ALMA Consulting Group, and we would like to warmly thank Drs Kosta Skarvelakis and Jetta Keranen for their professionalism and for having given us precious advice for making the project proposal a success, thanks to their deep expertise. ProBioGEM received financial support from the Université Lille 1, Sciences et Technologies, ARCIR funds from Nord-Pas-de-Calais, ANR (BtSurf) and the European Funds of INTERREG IV PhytoBio Project, ERA-IB MESIAB and ITN Marie Curie AMBER 317338.

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  1. Equipe Procédés Biologiques, Génie Enzymatique et Microbien, ProBioGEM, Institut Charles Viollette, E.A. 7394, Université de Lille et Université d’Artois, 59655, Villeneuve d’Ascq, France

    Philippe Jacques, Max Béchet, Muriel Bigan, Delphine Caly, Gabrielle Chataigné, François Coutte, Christophe Flahaut, Egon Heuson, Valérie Leclère, Didier Lecouturier, Vincent Phalip, Rozenn Ravallec, Pascal Dhulster & Rénato Froidevaux

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  1. Philippe Jacques
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Jacques, P., Béchet, M., Bigan, M. et al. High-throughput strategies for the discovery and engineering of enzymes for biocatalysis. Bioprocess Biosyst Eng 40, 161–180 (2017). https://doi.org/10.1007/s00449-016-1690-x

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