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Circ2388 regulates myogenesis and muscle regeneration

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Abstract

The formation of skeletal muscle is a complex process that is coordinated by many regulatory factors, such as myogenic factors and noncoding RNAs. Numerous studies have proved that circRNA is an indispensable part of muscle development. However, little is known about circRNAs in bovine myogenesis. In this study, we discovered a novel circRNA, circ2388, formed by reverse splicing of the fourth and fifth exons of the MYL1 gene. The expression of circ2388 was different between fetal and adult cattle muscle. This circRNA is 99% homologous between cattle and buffalo and is localized in the cytoplasm. Thoroughly, we proved that circ2388 had no effect on cattle and buffalo myoblast proliferation but promotes myoblast differentiation and myotube fusion. Furthermore, circ2388 in vivo stimulated skeletal muscle regeneration in mouse muscle injury model. Taken together, our findings suggest that circ2388 promotes myoblast differentiation and promotes the recovery and regeneration of damaged muscles.

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Data availability

The datasets used or analyzed in this study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the China Postdoctoral Science Foundation (Grant No. 2019M663842), the National Natural Science Foundation of China (Grant Nos. 32102514 and U20A2051), the Guangxi Natural Science Foundation (Grant Nos. 2019GXNSFAA185056 and 2020GXNSFBA297148), and the Science and Technology Base and Talent Special in Guangxi (Grant No. AD20159062).

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Authors and Affiliations

  1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, 712100, Xianyang, Yangling, Shaanxi, China

    Dandan Zhong, Yudong Cai, Hui Li & Yu Jiang

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources , College of Animal Science and Technology, Guangxi University, 530004, Nanning, China

    Dandan Zhong, Kongwei Huang, Liyin Zhang, Qingyou Liu, Deshun Shi & Hui Li

  3. Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, 528225, Foshan, China

    Qingyou Liu

  4. Animal Husbandry Station of Chongzuo City, 532200, Chongzuo, China

    Huiren Li

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  1. Dandan Zhong
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  2. Kongwei Huang
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  3. Liyin Zhang
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  9. Yu Jiang
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Correspondence to Hui Li.

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Ethical approval

The animal care and research protocol for this experiment has been approved by the Animal Care Committee of the College of Animal Science and Technology of Guangxi University.

Consent to participate

Dandan Zhong, Kongwei Huang, and Liyin Zhang were responsible for the execution of the experiment and drawing diagrams. Dandan Zhong and Kongwei Huang participated in the writing of the manuscript. Yudong Cai and Huiren Li participated in the analysis of the data. Qingyou Liu and Deshun Shi were involved in the revision of the manuscript. Hui Li and Yu Jiang participated in the experimental design and guidance. Dandan Zhong and Kongwei Huang contributed equally to this work. All authors read and approved the final manuscript.

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The authors declare no competing interests.

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Zhong, D., Huang, K., Zhang, L. et al. Circ2388 regulates myogenesis and muscle regeneration. Cell Tissue Res 393, 149–161 (2023). https://doi.org/10.1007/s00441-023-03787-1

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