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Role of sialylation of N-linked glycans in prion pathogenesis

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Abstract

Mammalian prion or PrPSc is a proteinaceous infectious agent that consists of a misfolded, self-replicating state of the prion protein or PrPC. PrPC and PrPSc are posttranslationally modified with N-linked glycans, which are sialylated at the terminal positions. More than 30 years have passed since the first characterization of the composition and structural diversity of N-linked glycans associated with the prion protein, yet the role of carbohydrate groups that constitute N-glycans and, in particular, their terminal sialic acid residues in prion disease pathogenesis remains poorly understood. A number of recent studies shed a light on the role of sialylation in the biology of prion diseases. This review article discusses several mechanisms by which terminal sialylation dictates the spread of PrPSc across brain regions and the outcomes of prion infection in an organism. In particular, relationships between the sialylation status of PrPSc and important strain-specific features including lymphotropism, neurotropism, and neuroinflammation are discussed. Moreover, emerging evidence pointing out the roles of sialic acid residues in prion replication, cross-species transmission, strain competition, and strain adaptation are reviewed. A hypothesis according to which selective, strain-specified recruitment of PrPC sialoglycoforms dictates unique strain-specific disease phenotypes is examined. Finally, the current article proposes that prion strains evolve as a result of a delicate balance between recruiting highly sialylated glycoforms to avoid an “eat-me” response by glia and limiting heavily sialylated glycoforms for enabling rapid prion replication.

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Fig. 1
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modified from Baskakov et al. (2018)

Fig. 4

Adapted from Baskakov and Katorcha (2016)

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Acknowledgements

We thank Kara Molesworth for editing this manuscript.

Funding

This work was supported by NIH grants NS045585 and AI128925.

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  1. Center for Biomedical Engineering and Technology and Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA

    Natallia Makarava & Ilia V. Baskakov

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Makarava, N., Baskakov, I.V. Role of sialylation of N-linked glycans in prion pathogenesis. Cell Tissue Res 392, 201–214 (2023). https://doi.org/10.1007/s00441-022-03584-2

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