Abstract
Sperm carries a reservoir of proteins regulating the molecular functions to attain functional competence. Semen samples collected from buffalo bulls were assessed for sperm functional attributes (n = 11) and proteome profiling (n = 6). Sperm proteins were extracted and profiled by employing LC–MS/MS. Overall, the buffalo sperm contained 1365 proteins, of which 458 were common between the groups. The unique proteins were 477 and 430 in good and poor quality semen, respectively. In the whole proteome of buffalo sperm, sexual reproduction with phosphatidylethanolamine-binding protein1 (PEBP1), fetuin-B (FETUB) and acrosin (ACR) was the most enriched (p = 8.44E−19) biological process, also with thermogenesis (p = 0.003), oocyte meiosis (p = 0.007) and vascular smooth muscle contraction (p = 0.009) apart from metabolic pathways. In good quality semen, mesenchyme migration (p = 1.24E−07) and morphogenesis (p = 0.001) were abundant biological processes. In good quality semen, the fluid shear stress (p = 0.01) and, in poor quality semen, valine, leucine and isoleucine degradation (p = 3.8E−05) pathways were enriched. In good quality semen, 7 proteins were significantly (p < 0.05) upregulated and 33 proteins were significantly (p < 0.05) downregulated. On validating the abundantly expressed sperm proteins, serine protease inhibitor Kazal-type 2-like (SPINK2; 2.17-fold) and neddylin (NEDD8; 1.13-fold) were upregulated and YBX2 was downregulated (0.41-fold) in good quality semen as compared with poor quality semen (1-fold). The present findings revealed the importance of sperm proteins in oocyte maturation, fertilization process and early embryonic development. The variations in the proteomic composition can be used as potential markers for the selection of breeding bulls.
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24 February 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00441-021-03417-8
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Acknowledgments
The study was supported by ICAR-All India Coordinated Research Project on “Nutritional and physiological interventions for enhancing reproductive performance in animals.” The authors sincerely acknowledge the support of the Director, ICAR-NIANP, Bangalore, and Coordinator AICRP for conducting research at ICAR-NIANP. The authors sincerely thank Dr. J.P Ravindra, Former Head, Animal Physiology Division, ICAR-NIANP for the technical inputs and critical reviewing of the manuscript. Dr. S. Selvaraju is supported by ICAR-National Fellow Project, ICAR, Ministry of Agriculture, Government of India.
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BBK, SS and NSKG: designed the experiment; ASS, RL and SD: performed experiments; RA: data interpretation; BBK, SS, ASS and NSKG: wrote the paper; RB: critically discussed the data; BBK, SS, NSKG and DTP: critically discussed the data, carried out data interpretation and prepared manuscript. All authors reviewed the manuscript.
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Binsila, B.K., Archana, S.S., Ramya, L. et al. Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality. Cell Tissue Res 383, 881–903 (2021). https://doi.org/10.1007/s00441-020-03303-9
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DOI: https://doi.org/10.1007/s00441-020-03303-9