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Distributions and relationships of chemically defined enteroendocrine cells in the rat gastric mucosa

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Abstract

This paper provides quantitative data on the distributions of enteroendocrine cells (EEC), defined by the hormones they contain, patterns of colocalisation between hormones and EEC relations to nerve fibres in the rat gastric mucosa. The rat stomach has three mucosal types: non-glandular stratified squamous epithelium of the fundus and esophageal groove, a region of oxyntic glands in the corpus, and pyloric glands of the antrum and pylorus. Ghrelin and histamine were both contained in closed cells, not contacting the lumen, and were most numerous in the corpus. Gastrin cells were confined to the antrum, and 5-hydroxytryptamine (5-HT) and somatostatin cells were more frequent in the antrum than the corpus. Most somatostatin cells had basal processes that in the antrum commonly contacted gastrin cells. Peptide YY (PYY) cells were rare and mainly in the antrum. The only numerous colocalisations were 5-HT and histamine, PYY and gastrin and gastrin and histamine in the antrum, but each of these populations was small. Peptide-containing nerve fibres were found in the mucosa. One of the most common types was vasoactive intestinal peptide (VIP) fibres. High-resolution analysis showed that ghrelin cells were closely and selectively approached by VIP fibres. In contrast, gastrin cells were not selectively innervated by VIP or CGRP fibres. The study indicates that there are distinct populations of gastric EEC and selective innervation of ghrelin cells. It also shows that, in contrast to EEC of the small intestine, the majority of EEC within the stomach contained only a single hormone.

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Acknowledgements

We thank Josiane Fakhry for helpful comments on the manuscript.

Funding

This work was supported by NIH (SPARC) grant ID # OT2OD023847 (PI Terry Powley) to JBF.

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Authors and Affiliations

  1. Department of Anatomy and Neuroscience, University of Melbourne, Parkville, Victoria, 3010, Australia

    Billie Hunne, Martin J. Stebbing, Rachel M. McQuade & John B. Furness

  2. Florey Institute of Neuroscience and Mental Health, Parkville, Victoria, 3010, Australia

    Martin J. Stebbing, Rachel M. McQuade & John B. Furness

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  1. Billie Hunne
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Hunne, B., Stebbing, M.J., McQuade, R.M. et al. Distributions and relationships of chemically defined enteroendocrine cells in the rat gastric mucosa. Cell Tissue Res 378, 33–48 (2019). https://doi.org/10.1007/s00441-019-03029-3

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