Abstract
Clinical studies have established the important impact of atherosclerotic disease in Western societies. This disease is characterized by the accumulation of lipids and the migration of various cell types in the sub-endothelial space of blood vessels. As demonstrated by many studies, endothelial cells play an essential role in the development of this disease. The endothelium acts as a gatekeeper of blood vessel integrity and cardiovascular health status. For instance, the transfer of lipids via the transport of lipoproteins in the arterial intima is believed to be mediated by endothelial cells through a process termed transcytosis. In addition, lipoproteins that accumulate in the sub-endothelial space may also be modified, in a process that can direct the activation of endothelial cells. These steps are essential for the initiation of an atherosclerotic plaque and may be mediated, at least in part, by caveolae and their associated protein caveolin-1. In the present study, we evaluate the role of caveolin-1/caveolae in the regulation of these two steps in endothelial cells. Our data clearly demonstrate that caveolin-1 is involved in the regulation of lipoprotein transcytosis across endothelial cells and in the regulation of vascular inflammation.
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Acknowledgments
The authors would like to thank Dr. Iset Medina Vera for her technical support. PGF was supported by grants from the Jane Barsumian/Mary Lyons Trust and the W.W. Smith Trust Fund. MPL was supported by grants from the National Institutes of Health and the American Heart Association. The Bioimaging Shared Resource of the Kimmel Cancer Center (NCI 5 P30 CA-56036) was used in this study.
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Pavlides, S., Gutierrez-Pajares, J.L., Iturrieta, J. et al. Endothelial caveolin-1 plays a major role in the development of atherosclerosis. Cell Tissue Res 356, 147–157 (2014). https://doi.org/10.1007/s00441-013-1767-7
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DOI: https://doi.org/10.1007/s00441-013-1767-7