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Expression and immunohistochemical localization of TMEM16A/anoctamin 1, a calcium-activated chloride channel in the mouse cochlea

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Abstract

Sound transduction in the cochlea depends on the unique high concentrations of K+ in the endolymph. The production and maintenance of high K+ concentrations are accompanied by Cl- cycling. In this study, we report on an investigation of the expression and localization of TMEM16A/anoctamin 1 (ANO1), a recently cloned Ca2+-activated Cl- channel, in the mouse cochlea by Western blot and immunhistochemistry. The ANO1 protein was identified in the cochlea by Western blotting. The immunoreactivity was found in stria vascularis as a line and in the organ of Corti as three plaques. The cellular localization of ANO1 was examined by means of double-labeling experiments with anti-claudin 11, a marker for basal cells of the stria vascularis. The results demonstrated that ANO1 colocalized with claudin 11, indicating its expression in basal cells. We also examined ANO1 localization in the organ of Corti by double- and triple-labeling techniques with anti-myosin VI, a marker for hair cells, and anti-synaptophysin, a marker for olivocochlear efferent nerve endings under hair cells. The results clearly showed that ANO1 is colocalized with synaptophysin, but not with myosin VI, indicating that ANO1 is localized at medial olivocochlear efferent nerve endings under outer hair cells. These results suggest that ANO1 may be specifically involved in synaptic transmission from medial olivocochlear efferent nerve endings to outer hair cells in the organ of Corti, as well as Cl- cycling in basal cells of the stria vascularis.

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Acknowledgement

We wish to thank Prof. Jinwoong Bok (Yonsei University) for his valuable comments.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul, 137–701, South Korea

    Ji Hyun Jeon, Jong Woo Lee, Sung Won Jeong & In-Beom Kim

  2. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul, 137–701, South Korea

    Jae Woo Park & Sang Won Yeo

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  1. Ji Hyun Jeon
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  2. Jae Woo Park
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  3. Jong Woo Lee
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Correspondence to Sang Won Yeo or In-Beom Kim.

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Ji Hyun Jeon and Jae Woo Park contributed equally to this work.

This study was supported by Basic Science Research Program (2010–0022317) and Medical Research Center Grant (R13-2002-005-01002-0) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology.

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Jeon, J.H., Park, J.W., Lee, J.W. et al. Expression and immunohistochemical localization of TMEM16A/anoctamin 1, a calcium-activated chloride channel in the mouse cochlea. Cell Tissue Res 345, 223–230 (2011). https://doi.org/10.1007/s00441-011-1206-6

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  • DOI: https://doi.org/10.1007/s00441-011-1206-6

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