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Importance of region-specific epithelial rearrangements in mouse rugae development

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Abstract

Epithelial appendages on palatal rugae develop during mouse palatogenesis through epithelial thickening and pattern formation. Recently, the patterned formation of nine rugae was observed together with the specific expression patterns of Shh in rodents. However, no crucial evidence was found for a direct association between Shh expression and the distinct structural formation of rugae. In order to reveal possible relationships, we investigated the morphological changes of rugae and expression patterns of Shh directly by in vitro organ culture at embryonic day 13 (E13) for 2 days. To compare and examine the diverse growing aspects of the palate and rugae, we carefully observed the detailed morphogenesis, with cell proliferation of the rugae occurring between E13 and E14.5. After 2 days of cultivation at E13, DiI micro-injections revealed that the middle part of the palate, adjacent to the upper molar-forming region, contributed to the formation of the subsequent structure of rugae by extensive cell rearrangement and proliferation within the epithelium in the preferred anteroposterior direction. The results also defined the intimate relationship between Shh expression and rugae formation.

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Authors and Affiliations

  1. School of Life Science and Biotechnology, Kyungpook National University, Daegu, Korea

    Wern-Joo Sohn, Zae Young Ryoo & Sanggyu Lee

  2. Department of Histology, Cytology and Developmental Anatomy, Nihon University School of Dentistry, Matsudo, Chiba, Japan

    Hitoshi Yamamoto

  3. Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea

    Hong-In Shin

  4. Department of Oral Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu, Korea

    Yong-Chul Bae

  5. Division of Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea

    Han-Sung Jung

  6. Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, 101 Dongin-dong 2ga, Joong-gu, Daegu, 700-422, Korea

    Jae-Young Kim

Authors
  1. Wern-Joo Sohn
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  2. Hitoshi Yamamoto
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  3. Hong-In Shin
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  4. Zae Young Ryoo
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  5. Sanggyu Lee
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  6. Yong-Chul Bae
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  7. Han-Sung Jung
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  8. Jae-Young Kim
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Corresponding author

Correspondence to Jae-Young Kim.

Additional information

Wern-Joo Sohn and Hitoshi Yamamoto contributed equally to this work.

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (no. 2010-0029494).

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Sohn, WJ., Yamamoto, H., Shin, HI. et al. Importance of region-specific epithelial rearrangements in mouse rugae development. Cell Tissue Res 344, 271–277 (2011). https://doi.org/10.1007/s00441-011-1148-z

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  • DOI: https://doi.org/10.1007/s00441-011-1148-z

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