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Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

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Abstract

We have previously reported the early uptake and transport of foreign particles into Peyer’s patches (PPs) of newborn and 2-month-old calves and shown that the peak uptake of particles occurs 6 h after inoculation, in addition to site- and size-related effects on particle uptake. We now report the distribution of immune cells within PPs of the distal ileum in newborn and 2-month-old calves inoculated with carbon black. The types of immune cells involved in the early uptake and transport of recombinant mouse prion protein (rMPrP) within PPs of newborn calf were investigated by using monoclonal antibodies CD11c, CD14, CD68, CD172a, and CD21. CD11c+, CD14+, CD68+, CD172a+, and CD21+ immune cells were widely distributed in four tissue compartments (villi, dome, interfollicular region, and follicles) of PPs in the distal ileum of newborn and 2-month-old calves, whereas CD11c+, CD14+, CD172a+, and CD21+ immune cells were more prominently distributed in the dome areas of newborn calves than in 2-month-old calves. Moreover, CD11c+ and CD14+ dendritic cells, CD172a+ and CD68+ macrophages, and CD21+ follicular dendritic cells containing rMPrP were primarily observed in the dome and inner follicular regions. The deposition of rMPrP within CD11c+, CD14+, CD172a+, and CD68+ cells, but not CD21+ cells, was detected in villous regions. rMPrP-positive immune cells within the interfollicular regions included only CD11c+ and CD172+ cells. Although the particles used in this investigation do not include the infectious prion protein, PrPSc, our experimental setup provides a useful model for studying immune cells involved in the early uptake and transport of PrPSc.

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Authors and Affiliations

  1. Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Yasuo Inoshima & Naotaka Ishiguro

  2. Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan

    Sein Lwin

  3. Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Yasuro Atoji

  4. Laboratory of Small Animal Surgery I, Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyoudai-Midorimachi 582, Ebetsu, 069-8501, Japan

    Hiroshi Ueno

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  1. Sein Lwin
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  2. Yasuo Inoshima
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  3. Yasuro Atoji
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  4. Hiroshi Ueno
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  5. Naotaka Ishiguro
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Correspondence to Naotaka Ishiguro.

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This study was partly supported by a grant for BSE research from Ministry of Health, Labour and Welfare, Japan (17270701) and a Grant-in-Aid (no. 17380180) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Lwin, S., Inoshima, Y., Atoji, Y. et al. Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves. Cell Tissue Res 338, 343–354 (2009). https://doi.org/10.1007/s00441-009-0879-6

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  • DOI: https://doi.org/10.1007/s00441-009-0879-6

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