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Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis

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Abstract

Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.

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Acknowledgements

We thank Ulf Gurok and Rogier Versteeg for critically reading the manuscript and Bettina Lipkowitz for excellent technical support. We also acknowledge the help of Tobias Müller in implementing the EM algorithm.

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  1. , Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, 14195, Berlin, Germany

    Georg Wieczorek, Christine Steinhoff, Ralph Schulz, Martin Vingron, H.-Hilger Ropers & Ulrike A. Nuber

  2. Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany

    Georg Wieczorek

  3. Research Facility for Experimental Medicine, Freie Universität Berlin, Krahmerstr. 6, 12207, Berlin, Germany

    Marina Scheller

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  1. Georg Wieczorek
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  2. Christine Steinhoff
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  3. Ralph Schulz
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Correspondence to Ulrike A. Nuber.

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This work was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 577, Teilprojekt C3)

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Wieczorek, G., Steinhoff, C., Schulz, R. et al. Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis. Cell Tissue Res 311, 227–237 (2003). https://doi.org/10.1007/s00441-002-0671-3

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