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OsMADS22, an STMADS11-like MADS-box gene of rice, is expressed in non-vegetative tissues and its ectopic expression induces spikelet meristem indeterminacy

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Abstract

We report the cDNA sequence and gene expression patterns of OsMADS22, a novel member of the STMADS11-like family of MADS-box genes, from rice. In contrast to previously reported STMADS11-like genes, whose expression is detected in vegetative tissues, OsMADS22 is mainly expressed during embryogenesis and flower development. In situ hybridization analysis revealed that OsMADS22 expression is localized in the L1 layer of embryos and in developing stamen primordia. Ectopic expression of OsMADS22 in transgenic rice plants resulted in aberrant floral morphogenesis, characterized by a disorganized palea, an elongated glume, and a two-floret spikelet. The results are discussed in terms of rice spikelet development and a novel non-vegetative role for a STMADS11-like gene.

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Acknowledgements

We are grateful to Dr. Seiichi Toki for providing the pUBA plasmid and to Dr. Masaharu Kuroda for providing the pZH2 binary vector. We appreciate Dr. Dale Karlson’s careful reading of the manuscript. This work was supported by grants from the Ministry of Agriculture, Forestry, and Fisheries (Rice Genome Project MP2117b) to R.I. and by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists to N.S.

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Authors and Affiliations

  1. National Agricultural Research Center for Hokkaido Region, Toyohira, Sapporo, 062-8555, Japan

    Naoki Sentoku, Hideki Kato & Ryozo Imai

  2. Department of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

    Naoki Sentoku & Hidemi Kitano

  3. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan

    Naoki Sentoku

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  1. Naoki Sentoku
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  2. Hideki Kato
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Correspondence to Naoki Sentoku.

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Sentoku, N., Kato, H., Kitano, H. et al. OsMADS22, an STMADS11-like MADS-box gene of rice, is expressed in non-vegetative tissues and its ectopic expression induces spikelet meristem indeterminacy. Mol Genet Genomics 273, 1–9 (2005). https://doi.org/10.1007/s00438-004-1093-6

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  • DOI: https://doi.org/10.1007/s00438-004-1093-6

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