Abstract
Chagas disease, endemic from Latin America, is caused by Trypanosoma cruzi and is transmitted by triatomine feces. This parasite undergoes complex morphological changes through its life cycle, promoted by significant changes in signal transduction pathways. The activity of protein kinase CK2 has been described in trypanosomatids. Using a specific peptide and radioactive ATP, we identified CK2 activity on the cellular surface and the cytoplasmic content in Trypanosoma cruzi, apart from the secreted form. Dephosphorylated casein promoted an increase of 48% in the secreted CK2 activity. Total extract of peritoneal macrophages from BALB/c and inactivated human serum promoted an increase of 67% and 36%, respectively, in this activity. The protein secreted by parasites was purified by HPLC and had shown compatibility with the catalytic subunit of mammalian CK2. Incubation of the parasites with CK2 inhibitors, added to the culture medium, prevented their growth. The opposite was observed when CK2 activators were used. Results of interaction between Trypanosoma cruzi and the gut of the vector have revealed that, in the presence of CK2 inhibitors, there is a reduction in the association rate. A similar inhibition profile was seen in the Trypanosoma cruzi-macrophages interaction, confirming the importance of this enzyme in the life cycle of this protozoan.
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This work was supported by FAPERJ No. 26/2021—Basic Research Grant (APQ1) in the State ICTs UERJ, UENF, and UEZO (E-26/211.845/2021); FAPERJ No. 31/2014—Support for Animal Facility Infrastructure in Teaching and Research Institutions Headquartered in RJ (E-26/210.553/2014), Edital FAPERJ No. 10/2014—Support Program for the Study of Neglected and Reemerging Diseases (E-26/210.053/2014).
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PMLD, JEOS, SAGS, AHCSL, MACSN, and GCA conceptualized the study and designed the experiments (methodology); JEOS, AKCL, SMRG, ACSB, and PMLD carried out the experiments; PMLD, JEOS, GCA, and AHCSL analyzed the results; PMLD and JEOS prepared the figures; PMLD and JEOS wrote the main manuscript (original draft); PMLD, AHCSL, and SAGS reviewed and edited the manuscript; PMLD supervised project direction and project administration. All authors contributed to the article and approved the submitted version.
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Peritoneal mouse macrophages used in this study were obtained in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Brazilian National Council of Animal Experimentation. The protocol was approved by the Ethics Committee on Animal Use (CEUA) of the Instituto de Biologia Roberto Alcântara Gomes of the Universidade do Estado do Rio de Janeiro (IBRAG)-UERJ, by the protocol numbers CEUA 047/2017 and CEUA 050/2017.
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de Oliveira Souza, J.E., Gomes, S.M.R., Lima, A.K.C. et al. Influence of CK2 protein kinase activity on the interaction between Trypanosoma cruzi and its vertebrate and invertebrate hosts. Parasitol Res 123, 80 (2024). https://doi.org/10.1007/s00436-023-08085-x
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DOI: https://doi.org/10.1007/s00436-023-08085-x