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Paragonimus heterotremus Chen et Hsia, 1964 (Digenea: Paragonimidae): species identification based on the biological and genetic criteria, and pathology of infection

  • Genetics, Evolution, and Phylogeny - Original Paper
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Abstract

As a result of the experimental infection of rats with metacercariae of Paragonimus heterotremus Chen et Hsia, 1964 from crabs (Potamiscus tannanti) caught in Yen Bai province, Vietnam, it was found that worms migrated into the lungs, to the liver and less frequently to the tissue that lines body cavities of the hosts, where they reached the adult stage, but in the muscles, worms stayed at the larval stage. Studies have shown that for P. heterotremus, rats can simultaneously play the role of the final and paratenic host; herewith, an infection with the trematode of this species can lead to the development of three forms of paragonimiasis: pulmonary, hepatic and muscular. Eggs from the adult worms localised in the liver, unlike eggs from the adult worms localised in the lungs, were not excreted into the external environment, but accumulated inside the organ. Histology and description of changes, which take place on the external surface of organs affected with P. heterotremus, are given in this study. Based on the behavioural characteristics of worms during rat infection and molecular genetic data, we established that worms from Vietnam and India should be assigned to different species of Paragonimus. P. heterotremus distribution is limited to the territory of the Southeast China, Northern Vietnam, Laos and Thailand.

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Funding

This work was partially supported by the FEB RAS (Far Eastern Branch of the Russian Academy of Sciences) project (18–4–039).

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Authors and Affiliations

  1. Federal Scientific Centre of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

    Anastasia N. Voronova, Vladimir V. Besprozvannykh & Yulia V. Tatonova

  2. Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam

    Ha Duy Ngo & Nguyen Manh Hung

  3. Pacific State Medical University, Ministry of Health of the Russian Federation, Vladivostok, Russia

    Natalia G. Plekhova

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  1. Anastasia N. Voronova
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  2. Vladimir V. Besprozvannykh
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  3. Ha Duy Ngo
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  4. Natalia G. Plekhova
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  5. Nguyen Manh Hung
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  6. Yulia V. Tatonova
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Correspondence to Anastasia N. Voronova.

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Voronova, A.N., Besprozvannykh, V.V., Ngo, H.D. et al. Paragonimus heterotremus Chen et Hsia, 1964 (Digenea: Paragonimidae): species identification based on the biological and genetic criteria, and pathology of infection. Parasitol Res 119, 4073–4088 (2020). https://doi.org/10.1007/s00436-020-06929-4

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