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Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis

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Abstract

Cathepsin C is an important exopeptidase of papain superfamily and plays a number of great important roles during the parasitic life cycle. The amino acid sequence of cathepsin C from Clonorchis sinensis (C. sinensis) showed 54, 53, and 49 % identities to that of Schistosoma japonicum, Schistosoma mansoni, and Homo sapiens, respectively. Phylogenetic analysis utilizing the sequences of papain superfamily of C. sinensis demonstrated that cathepsin C and cathepsin Bs came from a common ancestry. Cathepsin C of C. sinensis (Cscathepsin C) was identified as an excretory/secretory product by Western blot analysis. The results of transcriptional level and translational level of Cscathepsin C at metacercaria stage were higher than that at adult worms. Immunolocalization analysis indicated that Cscathepsin C was specifically distributed in the suckers (oral sucker and ventral sucker), eggs, vitellarium, intestines, and testis of adult worms. In the metacercaria, it was mainly detected on the cyst wall and excretory bladder. Combining with the results mentioned above, it implies that Cscathepsin C may be an essential proteolytic enzyme for proteins digestion of hosts, nutrition assimilation, and immune invasion of C. sinensis. Furthermore, it may be a potential diagnostic antigen and drug target against C. sinensis infection.

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Acknowledgments

This work was supported by National Key Basic Research and Development Project of China (973 project; No. 2010CB530000), National Natural Science Foundation of China (No. 81101270 and No. 81171602), National S & T Major Program (No. 2012ZX10004-220), and Fundamental Research Funds for the Central Universities (No. 3164035 and No. 3161036).

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Authors and Affiliations

  1. Department of Pathogen Biology, Hainan Medical College, Haikou, Hainan, 571199, China

    Pei Liang & Gang Lu

  2. Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Ministry of Education, Guangzhou, 510080, China

    Pei Liang, Lei He, Yanquan Xu, Xueqing Chen, Yan Huang, Mengyu Ren, Chi Liang, Xuerong Li, Jin Xu & Xinbing Yu

  3. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China

    Pei Liang, Lei He, Yanquan Xu, Xueqing Chen, Yan Huang, Mengyu Ren, Chi Liang, Xuerong Li, Jin Xu & Xinbing Yu

  4. Department of Clinical Laboratory, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou, 510220, China

    Lei He

  5. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510080, China

    Xueqing Chen

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  1. Pei Liang
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Correspondence to Gang Lu or Xinbing Yu.

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Pei Liang and Lei He contributed equally to this article.

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Liang, P., He, L., Xu, Y. et al. Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis . Parasitol Res 113, 3621–3629 (2014). https://doi.org/10.1007/s00436-014-4027-1

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