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Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA)

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Abstract

To know if alkaline phosphatase (AP) from schistosomes other than Schistosoma mansoni can be used as diagnostic marker for schistosomiasis in alkaline phosphatase immunocapture assay (APIA), we comparatively tested n-butanol extracts of adult worm membranes from a Venezuelan (JL) strain of S. mansoni (Ven/AWBE/Sm); a Cameroonian (EDEN) strain of Schistosoma intercalatum (Cam/AWBE/Si) and a Yemeni strain of Schistosoma haematobium (Yem/AWBE/Sh). APIA was evaluated with sera of patients from Venezuela, Senegal, and Gabon infected with S. mansoni, from Gabon infected with S. intercalatum or S. haematobium, from Chine infected with Schistosoma japonicum and from Cambodian patients infected with Schistosoma mekongi. Results indicate that 92.5 % (37/40) of Venezuela sera, 75 % (15/20) of Senegal sera, 39.5 % (17/43) of S. haematobium sera, and 19.2 % (5/26) S. intercalatum sera were APIA-positive with the Ven/AWBE/Sm preparation. APIA with the Cam/AWBE/Si preparation showed that 53.8 % of S. intercalatum-positive sera had anti-AP antibodies, and 51.2 % S. haematobium-positive sera cross-immunocapturing the S. intercalatum AP. APIA performed with Yem/AWBE/Sh showed that 55.8 % S. haematobium sera were positive. Only two out of nine S. japonicum sera were APIA-positive with the Ven/AWBE/Sm and Cam/AWBE/Si, and no reaction was observed with Cambodian S. mekongi-positive sera. AP activity was shown to be present in all the schistosome species/strains studied. The use of APIA as a tool to explore the APs antigenicity and the presence of Schistosoma sp. infections through the detection of anti-Schistosoma sp. AP antibodies in a host, allowed us to demonstrate the antigenicity of APs of S. mansoni, S. intercalatum, and S. haematobium.

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Acknowledgments

We thank the CNRS (France)-FONACIT (Venezuela) convention for the financial support of travels between France and Venezuela and ECOS-Nord V06A01. We thank César Matos and Anne Rognon for their contribution to maintenance of the life cycles of the schistosome species.

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Author notes

  1. I. M. Cesari

    Present address: Laboratório de Bioquímica e Biologia Molecular do Câncer, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - Prédio CCS - BL. E - sala 22, Ilha do Fundão - Cidade Universitária, Rio de Janeiro, RJ, CEP: 21941-902, Brazil

Authors and Affiliations

  1. Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas, 1020 A, Venezuela

    I. M. Cesari

  2. Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas, 1020 A, Venezuela

    D. E. Ballén & L. Mendoza

  3. Laboratoire de Parasitologie, Mycologie et Médecine Tropicale, U.F.R. de Médecine, 2bis, boulevard Tonnellé, 37032, Tours Cedex, France

    A. Ferrer & D. Richard-Lenoble

  4. USR 3278 CNRS-EPHE, Criobe Université de Perpignan, 52, Ave Paul Alduy, 66860, Perpignan, France

    J.-P. Pointier

  5. Département de Parasitologie–Mycologie–Médecine Tropicale, Faculté de Médecine et des Sciences de la Santé, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon

    M. Kombila

  6. CNRS, UMR 5244, Ecologie et Evolution des Interactions (2EI), Université de Perpignan Via Domitia, 52, Ave Paul Alduy, 66860, Perpignan Cedex, France

    A. Théron

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  1. I. M. Cesari
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Cesari, I.M., Ballén, D.E., Mendoza, L. et al. Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA). Parasitol Res 113, 1395–1403 (2014). https://doi.org/10.1007/s00436-014-3780-5

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  • DOI: https://doi.org/10.1007/s00436-014-3780-5

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