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Use of fluorescent lectin binding to distinguish Teladorsagia circumcincta and Haemonchus contortus eggs, third-stage larvae and adult worms

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Abstract

Lectin binding to carbohydrates on parasite surfaces has been investigated as a method of distinguishing adult worms, eggs and sheathed and exsheathed L3 of Teladorsagia circumcincta and Haemonchus contortus, economically important abomasal parasites in temperate climates. Both species were maintained as pure laboratory cultures of field isolates from New Zealand. Each of the four life cycle stages could be distinguished by the binding of at least one lectin: adult worms by Sambucus nigra agglutinin (SNA); eggs by peanut agglutinin (PNA), ConcavalinA and Lens culinaris agglutinin (LCA); exsheathed L3 by Griffonia simplicifolia-I lectin (GSL-I) and Lotus tetragonolobus lectin (LTL) and sheathed L3 by Aleuria aurantia lectin (AAL). The whole surface of both adult T. circumcincta and H. contortus strongly bound lectins specific for N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), mannose and fucose, but the two species could be distinguished by SNA binding only to T. circumcincta. Eggs could be distinguished by the binding of mannose-specific PNA to H. contortus and GalNAc-specific LCA and PSA to T. circumcincta eggs. GalNAc, GlcNAc and mannose lectins bound to the cuticle and over the excretory pores of a large proportion of sheathed L3 of both species, but only the H. contortus surface had exposed fucose or sialic acid complexes. The distinguishing lectin for sheathed L3 was AAL, which did not bind to T. circumcincta, but bound weakly to the head region of all fresh H. contortus and to 50–90% after 3 months storage. The cuticle of exsheathed L3 was unresponsive to all 19 lectins, and any binding was restricted to the head and tail regions. L3 exsheathed after 2–4 months storage could be distinguished by the binding of GSL-I and LTL to H. contortus but not to T. circumcincta. Lectin binding could be a useful adjunct in identifying L3, but lacked the consistency to be definitive, whereas it could be further developed as a practical method of distinguishing parasitic nematodes at other stages in the life cycle, particularly the eggs.

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Acknowledgements

We wish to thank Merial S.A.S. and Meat and Wool New Zealand for their generous financial support for this project. All marks ® are the property of their respective owners.

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Authors and Affiliations

  1. Institute for Parasitology, University of Veterinary Medicine, Buenteweg 17, 30559, Hannover, Germany

    Katharina Hillrichs & Thomas Schnieder

  2. Merial S.A.S, 29 Avenue Tony Garnier, Lyon, 69007, France

    Andrew B. Forbes

  3. Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, 4442, New Zealand

    David C. Simcock & Kevin C. Pedley

  4. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, 4442, New Zealand

    Heather V. Simpson

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  1. Katharina Hillrichs
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  2. Thomas Schnieder
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  3. Andrew B. Forbes
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  4. David C. Simcock
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  5. Kevin C. Pedley
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  6. Heather V. Simpson
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Correspondence to Heather V. Simpson.

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Hillrichs, K., Schnieder, T., Forbes, A.B. et al. Use of fluorescent lectin binding to distinguish Teladorsagia circumcincta and Haemonchus contortus eggs, third-stage larvae and adult worms. Parasitol Res 110, 449–458 (2012). https://doi.org/10.1007/s00436-011-2511-4

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