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Genotype identification of human cystic echinococcosis in Isfahan, central Iran

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Abstract

Echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of Iran with a major economic and public health importance. In the current study, Echinococcus granulosus isolates were collected from hospitalized patients in Isfahan, central Iran. The genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal DNA, followed by restriction fragment length polymorphism (RFLP) with two restriction enzymes namely AluI and MspI. As expected, each isolate yielded an approximately 1-kbp DNA fragment on the electrophoresis gel. According to RFLP results for both enzymes, all isolates had an equal pattern indicating the G1 genotype. Our findings confirmed that G1 is the dominant genotype of cystic echinococcosis in human in central Iran, with predilection to different organs including liver, lung, and brain, and warrants the importance of sheep dog cycle in public health.

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Acknowledgements

The authors would like to appreciate all the people who helped to perform this study including Mrs. N. Jalalizand for her kind contribution in molecular lab and the surgeons in Al-Zahra Hospital who had kind cooperation for collecting hydatid cysts samples. This study was financially supported by the National Institute of Health Research, Academic Pivot for Education and Research, Tehran University of Medical Sciences: Project No: 240/5142.

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Correspondence to Hossein Mirhendi.

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Kia, E.B., Rahimi, H., Sharbatkhori, M. et al. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res 107, 757–760 (2010). https://doi.org/10.1007/s00436-010-1947-2

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  • DOI: https://doi.org/10.1007/s00436-010-1947-2

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