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Comparison of preparation techniques of mixed samples (fungi–helminth eggs) for scanning electron microscopy by critical point drying

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Abstract

We compared three preparation techniques for critical point drying of fungus Paecilomyces lilacinus (Thom) Samson with Toxocara canis (Werner) Johnston and Taenia hydatigena Linneo eggs by scanning electron microscopy. We evaluated filtration (first), centrifugation (second), and phytoplankton network (third) in critical point drying methods. The first and third methods were advantageous for T. canis eggs because they preserved the quantity and quality of samples to obtain better images definition. The best technique for T. hydatigena eggs was the addition of phytoplankton network in critical point drying which preserved these helminth eggs.

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Acknowledgements

This work was made possible by the financial support from the Universidad Nacional de La Plata, Comisión de Investigaciones Científicas de la provincia de Buenos Aires, and the Agencia Nacional de Investigaciones Científicas y Técnicas.

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Authors and Affiliations

  1. Servicio de Microscopía Electrónica, Museo de Ciencias Naturales de La Plata, La Plata, Argentina

    P. L. Sarmiento

  2. Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calle 60 y 120 s/n, La Plata 1900, Argentina

    María L. Ciarmela, M. C. Minvielle & J. A. Basualdo

  3. Cátedra de Salud Pública, Departamento de Bioquímica, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia “San Juan Bosco”, Comodoro Rivadavia, Argentina

    P. Sánchez Thevenet

Authors
  1. P. L. Sarmiento
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  2. María L. Ciarmela
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  3. P. Sánchez Thevenet
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  4. M. C. Minvielle
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  5. J. A. Basualdo
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Correspondence to J. A. Basualdo.

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Sarmiento, P.L., Ciarmela, M.L., Thevenet, P.S. et al. Comparison of preparation techniques of mixed samples (fungi–helminth eggs) for scanning electron microscopy by critical point drying. Parasitol Res 99, 455–458 (2006). https://doi.org/10.1007/s00436-006-0187-y

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  • DOI: https://doi.org/10.1007/s00436-006-0187-y

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