Abstract
The damage to the tegument of 3-week-old Fasciola hepatica was evaluated by scanning electron microscopy (SEM) following treatment with the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole (called compound alpha) in its natural host. For the present study, flukes were raised in pelibuey sheep infected orally with metacercariae of F. hepatica; the parasites were recovered from the liver of the sacrificed sheep after 6, 12 and 24 h of treatment with compound alpha. At 6 h of treatment, the flukes showed some lesions on the ventral surface of the anterior region, such as a swollen tegument and blebs. At 12 h after treatment, the specimens showed structural disorganization and spine loss in the ventral anterior region. The tegument of the flukes treated for 24 h was completely lost in some areas of the ventral surface, leaving an exposed basal lamina. The tegument of immature F. hepatica might be a target organ for compound alpha to exert its fasciolicide effect.
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Acknowledgements
This research was supported by the project PAPIIT-DEGAPA-UNAM No. 1N214502-3. The authors would like to thank Francisco Pasos Najera for expert photographic assistance. This study complies with the current laws of Mexico.
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Rivera, N., Ibarra, F., Zepeda, A. et al. The effect of the 5-chloro-2-methylthio-6-(1-naphtyloxy)-1H-benzimidazole on the tegument of immature Fasciola hepatica in their natural host. Parasitol Res 95, 379–382 (2005). https://doi.org/10.1007/s00436-005-1304-z
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DOI: https://doi.org/10.1007/s00436-005-1304-z