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Entamoeba histolytica sequences and their relationship with experimental liver abscesses in hamsters

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Abstract

The purpose of the present paper was to analyse the association between sequences of Entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary DNA library for E. histolytica. From the sequences obtained, we designed oligonucleotides for amplification by PCR. Trophozoites were isolated from faeces of 11 patients in whom cysts from E. histolytica were identified, and these trophozoites were then subjected to monoaxenic culture. Then 1×105 trophozoites were inoculated into hamster liver, with three hamsters used for every culture. Sequences were obtained for ubiquitin, lectin surface precursor, replication factor MCM3 and surface antigen. The associations between sequences and hepatic abscesses were: 11/11 for ubiquitin, 9/11 for lectin precursor, 4/11 for replication factor and 1/11 for surface antigen. These results suggest that ubiquitin could be an important protein involved in the mechanism of E. histolytica invasion.

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References

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Ankri S, Stolarsky T, Mirelman D (1998) Antisense inhibition of expression of cysteine proteinases does not affect Entamoeba histolytica cytopathic or haemolytic activity but inhibits phagocytosis. Mol Microbiol 28:777–785

    Article  PubMed  CAS  Google Scholar 

  • Ankri S, Padilla-Vaca F, Stolarsky T, Koole L, Katz U, Mirelman D (1999) Antisense inhibition of expression of the light subunit (35 kDa) of the Gal/GalNac lectin complex inhibits Entamoeba histolytica virulence. Mol Microbiol 33:327–337

    Article  PubMed  CAS  Google Scholar 

  • Argiles JM, Pallares-Trujillo J, Lopez-Soriano FJ (1998) The role of the ubiquitin system in the etiology of cancer and other diseases. Med Clin (Barc) 11:423–426

    Google Scholar 

  • Bonilla E, del Mazo J (2003) Deregulation of gene expression in fetal oocytes exposed to doxorubicin. Biochem Pharmacol 65:1701–1707

    Article  PubMed  CAS  Google Scholar 

  • Bracha R, Nuchamowitz Y, Leippe M, Mirelman D (1999) Antisense inhibition of amoebapore expression in Entamoeba histolytica causes a decrease in amoebic virulence. Mol Microbiol 34:463–472

    Article  PubMed  CAS  Google Scholar 

  • Bruchhaus I, Loftus B, Hall N, Tannich E (2003) The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation. Eukaryot Cell 2:501–509

    Article  PubMed  CAS  Google Scholar 

  • Ciechanover A (1994) The ubiquitin–proteasome proteolytic pathway. Cell 79:13–21

    Article  PubMed  CAS  Google Scholar 

  • Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg 72:431–432

    Article  PubMed  CAS  Google Scholar 

  • Edman U, Meraz MA, Rausser S, Agabian N, Meza I (1990) Characterization of an immuno-dominant variable surface antigen from pathogenic and nonpathogenic Entamoeba histolytica. J Exp Med 172:879–888

    Article  PubMed  CAS  Google Scholar 

  • Finley D, Chau V (1991) Ubiquitination. Annu Rev Cell Biol 7:25–69

    Article  PubMed  CAS  Google Scholar 

  • Franco E, Manning-Cela R, Meza I (2002) Signal transduction in Entamoeba histolytica induced by interaction with fibronectin: presence and activation of phosphokinase A and its possible relation to invasiveness. Arch Med Res 33:389–397

    Article  PubMed  CAS  Google Scholar 

  • Gangopadhyay SS, Ray SS, Sinha P, Lohia A (1997) Unusual genome organisation in Entamoeba histolytica leads to two overlapping transcripts. Mol Biochem Parasitol 89:73–83

    Article  PubMed  CAS  Google Scholar 

  • Ghosh PK, Mancilla R, Ortiz-Ortiz L (1994) Intestinal amebiasis: histopathologic features in experimentally infected mice. Arch Med Res 25:297–302

    PubMed  CAS  Google Scholar 

  • Gonzalez J, Bai G, Frevert U, Corey EJ, Eichinger D (1999) Proteasome-dependent cyst formation and stage-specific ubiquitin mRNA accumulation in Entamoeba invadens. Eur J Biochem 264:897–904

    Article  PubMed  CAS  Google Scholar 

  • Jentsch S (1992) The ubiquitin-conjugation system. Annu Rev Genet 26:179–207

    Article  PubMed  CAS  Google Scholar 

  • Leippe M (1997) Amoebapores. Parasitol Today 13:178–183

    Article  PubMed  CAS  Google Scholar 

  • Mai J, Waisman DM, Sloane BF (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta 1477:215–230

    PubMed  CAS  Google Scholar 

  • Maniatis T, Frisch EF, Sambrook J (eds) (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

  • Mareel M, Leroy A (2003) Clinical, cellular, and molecular aspects of cancer invasion. Physiol Rev 83:337–376

    PubMed  CAS  Google Scholar 

  • Mattern CF, Keister DB (1977) Experimental amebiasis II. Hepatic amebiasis in the newborn hamster. Am J Trop Med Hyg 26:402–411

    PubMed  CAS  Google Scholar 

  • McIntyre GF, Godbold GD, Erickson AH (1994) The pH-dependent membrane association of procathepsin L is mediated by a 9-residue sequence within the propeptide. J Biol Chem 269:567–572

    PubMed  CAS  Google Scholar 

  • Petri WA Jr, Haque R, Lyerly D, Vines RR (2000) Estimating the impact of amebiasis on health. Parasitol Today 16:320–321

    Article  PubMed  Google Scholar 

  • Ravdin JI, Croft BY, Guerrant RL (1980) Cytopathogenic mechanisms of Entamoeba histolytica. J Exp Med 152:377–390

    Article  PubMed  CAS  Google Scholar 

  • Rawal S, Majumdar S, Vohra H (2005) Activation of MAPK kinase pathway by Gal/GalNAC adherence lectin of E. histolytica: gateway to host response. Mol Cell Biochem 268:93–101

    Article  PubMed  CAS  Google Scholar 

  • Robinson GL (1968) The laboratory diagnosis of human parasitic amoebae. Trans R Soc Trop Med Hyg 62:285–294

    Article  PubMed  CAS  Google Scholar 

  • Rodríguez MA, Orozco E, Arroyo R (1994) Proteins of Entamoeba histolytica trophozoites involved in the adhesion to target cells. Arch Med Res 25:205–210

    PubMed  Google Scholar 

  • Sloane BF, Rozhin J, Johnson K, Taylor H, Crissman J, Honn KV (1986) Cathepsin B: association with plasma membrane in metastatic tumors. Proc Natl Acad Sci U S A 83:2483–2487

    Article  PubMed  CAS  Google Scholar 

  • Sloane BF, Rozhin J, Hatfield JS, Crissman J, Honn KV (1987) Plasma membrane-associated cysteine proteinases in human and animal tumors. Exp Cell Biol 55:209–224

    PubMed  CAS  Google Scholar 

  • Staden Group (2003) Staden Package, version 2003.ob1. MRC Laboratory of Molecular Biology, Cambridge, UK

    Google Scholar 

  • Tannich E, Ebert F, Horstmann RD (1991) Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica. Proc Natl Acad Sci U S A 88:1849–1853

    Article  PubMed  CAS  Google Scholar 

  • Tavares P, Rigothier MC, Khun H, Roux P, Huerre M, Guillen N (2005) Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance. Infect Immun 73:1771–1778

    Article  PubMed  CAS  Google Scholar 

  • Treviño Garcia-Manzo N, Escandon-Romero C, Escobedo de la Pena J, Hernandez-Ramos JM, Fierro-Hernandez H (1994) Amebiasis in the epidemiologic transition in Mexico: its morbidity and mortality trends in the Mexican Institute of Social Security. Arch Med Res 25:393–399

    PubMed  Google Scholar 

  • Wells CD, Argueda M (2004) Amebic liver abscess. South Med J 97:673–682

    Article  PubMed  Google Scholar 

  • Wostmann C, Tannich E, Bakker-Grunwald T (1992) Ubiquitin of Entamoeba histolytica deviates in six amino acid residues from the consensus of all other known ubiquitins. FEBS Lett 308:54–58

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Parasitology Research, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162 Col. Doctores, 06720, Mexico, DF, Mexico

    María del Pilar Crisóstomo-Vázquez & Enedina Jiménez-Cardoso

  2. Universidad Autónoma de Querétaro, Querétaro, Mexico

    Carlos Arroyave-Hernández

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  1. María del Pilar Crisóstomo-Vázquez
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  2. Enedina Jiménez-Cardoso
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  3. Carlos Arroyave-Hernández
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Correspondence to Enedina Jiménez-Cardoso.

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Crisóstomo-Vázquez, M., Jiménez-Cardoso, E. & Arroyave-Hernández, C. Entamoeba histolytica sequences and their relationship with experimental liver abscesses in hamsters. Parasitol Res 98, 94–98 (2006). https://doi.org/10.1007/s00436-005-0006-x

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  • DOI: https://doi.org/10.1007/s00436-005-0006-x

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