Abstract
Polymerase chain reaction (PCR) for the identification of eggs of the tapeworm Echinococcus granulosus (“sheep strain”) was evaluated with primers derived from mitochondrial sequences. Specificity of these primers was confirmed by investigating DNA of other strains of E. granulosus and of 14 helminth species which inhabit the intestines of dogs. This PCR assay was used to investigate 131 purged dogs from Kazakhstan. Eighteen dogs harboured Echinococcus worms, ten of them in mixed infections with Taenia spp. Coproantigen detection was positive in 15 and taeniid eggs could be recovered from 13 of these specimens. Eight of the egg-containing samples were positive in the PCR for E. granulosus and four in a Echinococcus multilocularis -specific PCR revealing one mixed infection. Egg-containing faeces from two dogs harbouring both Taenia spp. and Echinococcus spp. were negative in both PCRs. The combination of egg isolation and PCR will also be of value in epidemiological studies when investigating environmental samples.
References
Abbasi I, Branzenburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig PS, Hamburger J (2003) Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. Am J Trop Med Hyg 69:324–330
Allan J, Craig PS, Gracie Noval J, Mencos F, Liu D, Wang Y, Wen H, Zhou P, Stringer R, Rogan M, Zeyhle E (1992) Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans. Parasitology 104:347–356
Bowles J, Blair D, McManus DP (1995) A molecular phylogeny of the genus Echinococcus. Parasitology 110:317–328
Cabrera M, Canova S, Rosenzvit M, Guarnera E (2002) Identification of Echinococcus granulosus eggs. Diagn Microbiol Infect Dis 44:29–34
Celi FS, Zenilman ME, Shuldiner AR (1993) A rapid and versatile method to synthesise internal standards for competitive PCR. Nucleic Acids Res 21:1047
Christofi G, Deplazes P, Christofi N, Tanner I, Economides P, Eckert J (2002) Screening of dogs for Echinococcus granulosus coproantigen in a low endemic situation in Cyprus. Vet Parasitol 104:299–306
Craig PS, Macpherson CN, Nelson GS (1986) The identification of eggs of Echinococcus by immunofluorescence using a specific anti-oncospheral monoclonal antibody. Am J Trop Med Hyg 35:152–158
Deplazes P, Gottstein B, Eckert J, Jenkins DJ, Ewald D, Jimenez-Palacios S (1992) Detection of Echinococcus coproantigens by enzyme-linked immunosorbent assay in dogs, dingoes and foxes. Parasitol Res 78:303–308
Deplazes P, Dinkel A, Mathis A (2003) Molecular tools for studies on the transmission biology of Echinococcus multilocularis. Parasitology (in press)
Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T (1998) Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol 36:1871–1876
Eckert J, Deplazes P (2003) Biological, epidemiological and clinical aspects of echinococcosis: a zoonoses of increasing concern. Clin Microbiol Rev (in press)
Eckert J, Schantz PM, Gasser RB, Torgerson PR, Bessonov AS, Movsessian SO, Thakur A, Grimm F, Nikogossian MA (2001a) Geographic distribution and prevalence. In: Eckert J, Gemmell MA, Meslin F-X, Pawlowski Z (eds) WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. Office International des Epizooties, Paris, pp 100–142
Eckert J, Deplazes P, Craig PS, Gemmell MA, Gottstein B, Heath D, Jenkins DJ, Kamiya M, Lightowlers M (2001b) Echinococcosis in animals: clinical aspects, diagnosis and treatment. In: Eckert J, Gemmell MA, Meslin F-X, Pawlowski Z (eds) WHO/OIE Manual on echinococcosis in humans and animals: a public health problem of global concern. Office International des Epizooties, Paris, pp 72–99
Fraser A, Elayoubi F, Craig PS (2002) Detection of cestode infections in definitive hosts: present status and future advances. In: Craig P, Pawlowski Z (eds) Cestode zoonoses: echinococcosis and cysticercosis, an emergent and global problem. IOS, Amsterdam, pp 157–175
Malgor R, Nonaka N, Basmadjian I, Sakai H, Carambula B, Oku Y, Carmona C, Kamiya M (1997) Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay. Int J Parasitol 27:1605–1612
Mathis A, Deplazes P (2002) Role of PCR-DNA detection of Echinococcus multilocularis. In: Craig P, Pawlowski Z (eds) Cestode zoonoses: echinococcosis and cysticercosis, an emergent and global problem. IOS, Amsterdam, pp 195–204
Mathis A, Deplazes P, Eckert J (1996) Improved test system for PCR-based specific detection of Echinococcus multilocularis eggs. J Helminthol 70:219–222
O’Neill SL, Giordano R, Colbert AME, Karr TL, Robertson HM (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci USA 89:2699–2702
Schantz PM, Chai J, Craig PS, Eckert J, Jenkins DJ, Macpherson CNL, Thakur A (1995) Epidemiology and control of hydatid disease. In: Thompson RCA, Lymbery AJ (eds) Echinococcus and hyatid disease. CAB International, Wallingford, Oxford, 233–331
Stieger C, Hegglin D, Schwarzenbach G, Mathis A, Deplazes P (2002) Spatial and temporal aspects of urban transmission of Echinococcus multilocularis. Parasitology 124:631–640
Thompson RCA, McManus DP (2002) Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol 18:452–457
Torgerson PR, Budke CM (2003) Echinococcosis—an international public health challenge. Res Vet Sci 74:191–202
Torgerson PR, Shaikenov BS, Baitursinov KK, Abdybekova AM (2002) The emerging epidemic of echinococcosis in Kazakhstan. Trans R Soc Trop Med Hyg 96:124–128
Torgerson PR, Karaeva RR, Corkeri N, Abdyjaparov TA, Kuttubaev OT, Shaikenov BS (2003) Human cystic echinococcosis in Kyrgystan: an epidemiological study. Acta Trop 85:51–61
Acknowledgements
We greatly acknowledge the financial support of INTAS (grant nos. 01 500 and 01 505) and of the Swiss National Science Foundation (SCOPES programme, grant no. 7KKPJO65622). This work represents the dissertation of S. Štefanić, veterinarian.
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Štefanić, S., Shaikenov, B.S., Deplazes, P. et al. Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (“sheep strain”) in naturally infected dogs. Parasitol Res 92, 347–351 (2004). https://doi.org/10.1007/s00436-003-1043-y
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DOI: https://doi.org/10.1007/s00436-003-1043-y