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Expression of claudin 10 protein in hepatocellular carcinoma: impact on survival

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Abstract

Purpose

To investigate the expression of claudin 10 in hepatocellular carcinoma (HCC) and the impact on angiogenesis and the postoperative survival of HCC patients.

Methods

The expression of claudin 10 protein was analyzed on samples from 99 HCC patients undergoing hepatectomy with immunohistochemistry and 31 fresh specimens with Western blotting. We examined the relationship between claudin 10 expression with clinicopathological factors, microvessel density (MVD), and postoperative survival.

Results

Western blotting and immunohistochemical staining showed that claudin 10 protein was highly expressed in HCC, compared with paraneoplastic liver tissue and normal liver tissue (P < 0.01). Claudin 10 protein expression levels were significantly higher in HCC specimens with microscopic venous invasion (P < 0.01). MVD in HCC increased with enhanced claudin 10 expression (P < 0.01). Kaplan–Meier survival analysis revealed that HCC patients with high claudin 10 expression had significantly shorter overall survival (P = 0.01). Multivariate analysis showed that claudin 10 expression was an independent prognostic indicator for postoperative overall survival of HCC patients (P < 0.01).

Conclusions

Claudin 10 protein is highly expressed in HCC tissue and is closely related to angiogenesis. Claudin 10 protein could be a useful marker to predict poor prognosis of HCC patients after hepatectomy.

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References

  • Aldred MA, Huang Y, Liyanarachchi S, Pellegata NS, Gimm O, Jhiang S, Davuluri RV, de la Chapelle A, Eng C (2004) Papillary and follicular thyroid carcinomas show distinctly different microarray expression profiles and can be distinguished by a minimum of five genes. J Clin Oncol 22(17):3531–3539

    Article  PubMed  CAS  Google Scholar 

  • Cheung ST, Leung KL, Ip YC, Chen X, Fong DY, Ng IO, Fan ST, So S (2005) Claudin-10 expression level is associated with recurrence of primary hepatocellular carcinoma. Clin Cancer Res 11(2 pt 1):551–556

    PubMed  CAS  Google Scholar 

  • Choi YL, Kim J, Kwon MJ, Choi JS, Kim TJ, Bae DS, Koh SS, In YH, Park YW, Kim SH, Ahn G, Shin YK (2007) Expression profile of tight junction protein claudin 3 and claudin 4 in ovarian serous adenocarcinoma with prognostic correlation. Histol Histopathol 22(11):1185–1195

    PubMed  CAS  Google Scholar 

  • Fan ST, Lo CM, Liu CL, Lam CM, Yuen WK, Yeung C, Wong J (1999) Hepatectomy for hepatocellular carcinoma: toward zero hospital death. Ann Surg 229(3):322–330

    Article  PubMed  CAS  Google Scholar 

  • He J, Gu D, Wu X, Reynolds K, Duan X, Yao C, Wang J, Chen CS, Chen J, Wildman RP, Klag MJ, Whelton PK (2005) Major causes of death among men and women in China. N Engl J Med 353(11):1124–1134

    Article  PubMed  CAS  Google Scholar 

  • Huang GW, Yang LY (2002) Metallothionein expression in hepatocellular carcinoma and its clinical significance. World J Gastroenterol 8(4):650–653

    PubMed  CAS  Google Scholar 

  • Huang GW, Yang LY, Lu WQ (2005) Expression of hypoxia-inducible factor 1 alpha and vascular endothelial growth factor in hepatocellular carcinoma: impact on neovascularization and survival. World J Gastroenterol 11(11):1705–1708

    PubMed  CAS  Google Scholar 

  • Huang GW, Tao YM, Ding X (2009) Endocan expression correlated with poor survival in human hepatocellular carcinoma. Dig Dis Sci 54(2):389–394

    Article  PubMed  CAS  Google Scholar 

  • Hubert C, Sempoux C, Rahier J, Horsmans Y, Geubel A, Van Beers BE, Annet L, Zech F, Leonard D, Giqot JF (2007) Prognostic risk factors of survival after resection of hepatocellular carcinoma. Hepatogastroenterology 54(78):1791–1797

    PubMed  Google Scholar 

  • Ip YC, Cheung ST, Lee YT, Ho JC, Fan ST (2007) Inhibition of hepatocellular carcinoma invasion by suppression of claudin- 10 in HLE cells. Mol Cancer Ther 6(11):2858–2867

    Article  PubMed  CAS  Google Scholar 

  • Li J, Chigurupati S, Agarwal R, Mughal MR, Mattson MP, Becker KG, Wood WH 3rd, Zhang Y, Morin PJ (2009) Possible angiogenic roles for claudin-4 in ovarian cancer. Cancer Biol Ther 8(19):1814–1822

    Google Scholar 

  • Mann CD, Neal CP, Garcea G, Manson MM, Dennison AR, Berry DP (2007) Prognostic molecular markers in hepatocellular carcinoma: a systematic review. Eur J Cancer 43(6):979–992

    Article  PubMed  CAS  Google Scholar 

  • Morita K, Tsukita S, Miyachi Y (2004) Tight junction-associated protein (occludin, ZO-1, claudin-1, claudin-4) in squamous cell carcinoma and Bowen’s disease. Br J Dermatol 151(2):328–334

    Article  PubMed  CAS  Google Scholar 

  • Németh Z, Szász AM, Tátrai P, Németh J, Gyorffy H, Somorácz A, Szíjártó A, Kupcsulik P, Kiss A, Schaff Z (2009) Claudin-1, -2, -3, -4, -7, -8, and–10 protein expression in biliary tract cancers. J Histochem Cytochem 57(2):113–121

    Article  PubMed  Google Scholar 

  • Nichols LS, Ashfaq R, Iacobuzio-Donahue CA (2004) Claudin 4 protein expression in primary and metastatic pancreatic caner: support for use as a therapeutic target. Am J Clin Pathol 121(2):226–230

    Article  PubMed  CAS  Google Scholar 

  • Rangel LB, Agarwal R, D’Souza T, Pizer ES, Alò PL, Lancaster WD, Gregoire L, Schwartz DR, Cho KR, Morin PJ (2003) Tight junction proteins claudin-3 and claudin-4 are frequently overexpressed in ovarian cancer but not in ovarian cystadenomas. Clin Cancer Res 9(7):2567–2575

    PubMed  CAS  Google Scholar 

  • Resnick MB, Konkin T, Routhier J, Sabo E, Pricolo VE (2005) Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study. Mod Pathol 18(4):511–518

    Article  PubMed  CAS  Google Scholar 

  • Sanada Y, Yoshida K, Itoh H (2007) Comparison of CT enhancement patterns and histologic features in hepatocellular carcinoma up to 2 cm: assessment of malignant potential with claudin-10 immunohistochemistry. Oncol Rep 17(5):1177–1182

    PubMed  Google Scholar 

  • Santin AD, Zhan F, Bellone S, Palmieri M, Cane S, Bignotti E, Anfossi S, Gokden M, Dunn D, Roman JJ, O’Brien TJ, Tian E, Cannon MJ, Shaughnessy J Jr, Pecorelli S (2004) Gene expression profiles in primary ovarian serous papillary tumors and normal ovarian epithelium: identification of candidate molecular markers for ovarian cancer diagnosis and therapy. Int J Cancer 112(1):14–25

    Article  PubMed  CAS  Google Scholar 

  • Sherman M (2008) Recurrence of hepatocellular carcinoma. N Engl J Med 359(19):2045–2047

    Article  PubMed  CAS  Google Scholar 

  • Sugita M, Geraci M, Gao B, Powell RL, Hirsch FR, Johnson G, Lapadat R, Gabrielson E, Bremnes R, Bunn PA, Franklin WA (2002) Combined use of oligonucleotide and tissue microarrays identifies cancer/testis antigens as biomarkers in lung carcinoma. Cancer Res 62(14):3971–3979

    PubMed  CAS  Google Scholar 

  • Tokes AM, Kulka J, Paku S, Szik A, Páska C, Novák PK, Szilák L, Kiss A, Bögi K, Schaff Z (2005) Claudin-1, -3 and -4 proteins and mRNA expression in benign and malignant breast lesions: a research study. Breast Cancer Res 7(2):R296–R305

    Article  PubMed  CAS  Google Scholar 

  • Wang W, Yang LY, Huang GW, Lu WQ, Yang ZL, Yang JQ, Liu HL (2004) Genomic analysis reveals RhoC as a potential marker in hepatocellular carcinoma with poor prognosis. Br J Cancer 90(12):2349–2355

    PubMed  CAS  Google Scholar 

  • Wang SM, Ooi LL, Hui KM (2007) Identification and validation of a novel gene signature associated with the recurrence of human hepatocellular carcinoma. Clin Cancer Res 13(21):6275–6283

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan Province, People’s Republic of China

    G. W. Huang

  2. Department of Organ Transplantation, Xiangya Hospital, Central South University, Changsha, 410008, People’s Republic of China

    X. Ding

  3. Department of Pathology, Hunan Provincial Tumor Hospital, Changsha, 410008, People’s Republic of China

    S. L. Chen & L. Zeng

Authors
  1. G. W. Huang
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  2. X. Ding
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  3. S. L. Chen
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  4. L. Zeng
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Corresponding author

Correspondence to G. W. Huang.

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Huang, G.W., Ding, X., Chen, S.L. et al. Expression of claudin 10 protein in hepatocellular carcinoma: impact on survival. J Cancer Res Clin Oncol 137, 1213–1218 (2011). https://doi.org/10.1007/s00432-011-0987-z

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  • DOI: https://doi.org/10.1007/s00432-011-0987-z

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