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Increased expression of cellular retinol-binding protein 1 in laryngeal squamous cell carcinoma

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Abstract

Purpose

To investigate the genomic alterations in larynx carcinomas (LaCa) tissues and its prognostics values in predicting survival.

Methods

To analyse the aberrations in the genome of LaCa patients, we used array comparative genomic hybridization in 19 human laryngeal tumour samples. DNA samples were also subjected to detect human papillomavirus (HPV) sequences by polymerase chain reaction (PCR). Copy number gain was confirmed by real-time PCR. The cellular retinol-binding protein 1 (CRBP-1) gene expression was also confirmed by immunohistochemistry assay on LaCa tissues. To identify prognostic feature, CRBP-1 gene gain was correlated to patient survival.

Results

The most common gains were detected for CRBP-1 and EGFR genes, while DNA lost in RAF-1 gene. Immunohistochemistry assay was revealed strong expression of CRBP1 protein in those cases with CRBP-1 gene gain. The CRBP-1 gene gain and its expression correlated significantly with survival (P = 0.003). Cox regression analysis indicated that CRBP-1 expression level was a factor of survival (P = 0.008). HPV sequences were detected in 42% of the samples, and did not show any relationship with specific gene alterations.

Conclusion

Our data shows that CRBP-1 gene gain can be determined by immunohistochemistry on routinely processed tissue specimens, and could support as a potential novel marker for long-term survival in laryngeal squamous cell carcinoma.

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Acknowledgments

This work was partially supported by 7114, 69719 and 87244 Fondos Sectoriales en SALUD grants from Mexican Council of Science and Technology (CONACyT). Raúl Peralta was recipient of scholarship from the CONACyT and IMSS.

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Authors and Affiliations

  1. Oncology Research Unit, Hospital Oncologia, Health Research Coordination, IMSS, Mexico City, Mexico

    Raúl Peralta, Guelaguetza Vazquez, Sergio Juárez, Alejandra Valdivia, Patricia Piña & Mauricio Salcedo

  2. Institute of Molecular Biology, University of Zurich, Zurich, Switzerland

    Michael Baudis

  3. Hospital CIMA-Santa Engracia, Monterrey, NL, Mexico

    Rocío Ortiz & Horacio Decanini

  4. Epidemiology Department, Hospital de Oncologia, Centro Medico Nacional SXXI-IMSS, Mexico City, Mexico

    Dulce Hernandez

  5. Head and Neck Department, Hospital de Oncologia, Centro Medico Nacional SXXI-IMSS, Mexico City, Mexico

    Francisco Gallegos

  6. Unidad de Investigación en Enfermedades Oncológicas, Hospital de Oncologia, CMN SXXI-IMSS, Av. Cuauhtemoc 330, Col Doctores, México DF, 06720, México

    Mauricio Salcedo

Authors
  1. Raúl Peralta
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  2. Michael Baudis
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  3. Guelaguetza Vazquez
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  4. Sergio Juárez
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  5. Rocío Ortiz
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  6. Horacio Decanini
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  7. Dulce Hernandez
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  8. Francisco Gallegos
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  9. Alejandra Valdivia
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  10. Patricia Piña
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  11. Mauricio Salcedo
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Corresponding author

Correspondence to Mauricio Salcedo.

Additional information

Raúl Peralta is a Ph.D. student at Programa de Biomedicina y Biotecnologia Molecular, ENCB-IPN.

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Peralta, R., Baudis, M., Vazquez, G. et al. Increased expression of cellular retinol-binding protein 1 in laryngeal squamous cell carcinoma. J Cancer Res Clin Oncol 136, 931–938 (2010). https://doi.org/10.1007/s00432-009-0735-9

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  • DOI: https://doi.org/10.1007/s00432-009-0735-9

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