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Human T lymphotropic virus type 1 in a seronegative B chronic lymphocytic leukemia patient

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Abstract

Human T lymphotropic virus type 1 (HTLV-1) is the etiological agent of adult T cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 infection in patients with B cell-type chronic lymphocytic leukemia (B-CLL) is rare and has been reported only in areas in which HTLV-1 is endemic. In the present study, we detected HTLV-1 proviral DNA by polymerase chain reaction, using tax primers, in peripheral blood lymphocytes from a B-CLL patient, an immigrant to Israel, where HTLV-1 infection is not endemic. F344 rats injected intravenously with peripheral blood lymphocytes obtained from the patient developed HTLV-1 antibodies. Titers of antibody to HTLV-1 in the rat blood were 1:512 by particle agglutination; enzyme-linked immunosorbent assay and Western blotting were also positive. No antibody against HTLV-1 was demonstrated in the animal model after inoculation of either purified B lymphocytes from the B-CLL patient or peripheral blood mononuclear cells from healthy donors. This is one of the few studies showing the presence of HTLV-1 provirus in T lymphocytes of a B-CLL patient who had multiple infections, and died of salmonella sepsis, and the first report of HTLV-1 antibody induction in an animal model by inoculation of lymphocytes obtained from an HTLV-1-infected B-CLL patient.

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Authors and Affiliations

  1. Institute of Hematology, Rabin Medical Center, Beilinson Campus, , 49100, Petah Tiqva, Israel

    Pinhas Stark, Jacob Luboshitz, Matityahu Shaklai & Batya Shohat

  2. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

    Pinhas Stark, Jacob Luboshitz, Matityahu Shaklai & Batya Shohat

  3. Deutsches Primatenzentrum, Göttingen, Germany

    Walter Bodemer & Horst Hannig

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  1. Pinhas Stark
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  2. Walter Bodemer
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  3. Horst Hannig
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  4. Jacob Luboshitz
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  5. Matityahu Shaklai
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  6. Batya Shohat
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Correspondence to Pinhas Stark.

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Stark, P., Bodemer, W., Hannig, H. et al. Human T lymphotropic virus type 1 in a seronegative B chronic lymphocytic leukemia patient. Med Microbiol Immunol 192, 205–209 (2003). https://doi.org/10.1007/s00430-002-0169-3

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  • DOI: https://doi.org/10.1007/s00430-002-0169-3

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