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An additional long-term mechanism of NF-κB regulation after cytokine treatment in a human hepatoma cell line

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Abstract

The eukaryotic transcription factor nuclear factor-κB (NF-κB) is mainly involved in the regulation of immune response and inflammation. A prolonged activation of NF-κB has been reported in context with chronic diseases. What leads to a prolongation of NF-κB activity is not well understood. Here, an increase in total intracellular NF-κB protein and mRNA levels as well as a temporary colocalization of NF-κB with proteasomes in human hepatocytes after treatment with TNF-α or IL-1β is reported. This indicates that beside an instantaneous activation of NF-κB and partly autoregulated inactivation by breakdown and synthesis of the inhibitors of NF-κB (IκBs), there are also mechanisms for a long-term regulation by de novo-synthesis and degradation of NF-κB protein.

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Acknowledgements

The author thanks Prof. Dr. H. Dariush Fahimi (Institute of Anatomy and Cell Biology II, University of Heidelberg) for the possibility to work on this topic, Prof. Dr. Fahimi and Prof. Dr. Dr. Alfred Völkl (Institute of Anatomy and Cell Biology II, University of Heidelberg) for the theoretical and technical advice, Ms. Gabriele Krämer (Institute of Anatomy and Cell Biology II, University of Heidelberg) for the excellent technical assistance, Dr. C. Kuhn (ZMBH, Heidelberg) for the HepG2 cells, Mr. Daniel Kühn for graphic expertise, and Dr. Dietmar Techel (Institute of Pathology, Klinikum Stuttgart) for the critical reading.

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  1. Patricia Kohlhof

    Present address: Institute of Pathology, Klinikum Stuttgart, Kriegsbergstrasse 60, 70174, Stuttgart, Germany

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  1. Institute of Anatomy and Cell Biology II, University of Heidelberg, Heidelberg, Germany

    Patricia Kohlhof

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Kohlhof, P. An additional long-term mechanism of NF-κB regulation after cytokine treatment in a human hepatoma cell line. Virchows Arch 457, 585–595 (2010). https://doi.org/10.1007/s00428-010-0970-0

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