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Interstitial cells of Cajal could regenerate and restore their normal distribution after disrupted by intestinal transection and anastomosis in the adult guinea pigs

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Abstract

Surgical manipulations of the gastrointestinal (GI) tract usually lead to loss of interstitial cells of Cajal (ICCs). The present study prepared to investigate whether ICCs can regenerate and restore their normal distribution up to 5 months after semitransection and end-to-end anastomosis of small intestines of adult guinea pigs. The segments of anastomosis were studied by immunohistochemistry with anti-KIT, 5-bromo-2′-deoxyuridine (BrdU), stem cell factor (SCF), and neurofilament 200 antibodies and also by transmission electron microscopy (TEM). At early stage, intestinal surgery led to intestinal wall impairment and ICCs loss, and ICCs near the site of anastomosis gradually increased in numbers. About 150 days postoperation, the distribution of ICCs and the microstructure of intestinal wall appeared to be similar with those of the control. By double immunostaining with BrdU and KIT antibodies, a number of proliferated ICCs were seen near the site of transection/anastomosis. Furthermore, KIT ligand, SCF, was mainly observed in the smooth muscle cells (SMCs), which are located close to ICCs. TEM observation revealed a number of immature and mature ICCs in this region. Our results indicated that ICCs could regenerate and restore their normal distribution after intestinal surgery and SMCs might be involved in the regenerated events of ICCs in the adult guinea pig GI tract.

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Abbreviations

DMP:

Deep muscular plexus

GI:

Gastrointestinal

ICCs:

Interstitial cells of Cajal

IM:

Intramuscular

MY:

Myenteric plexus

SCF:

Stem cell factor

SMCs:

Smooth muscle cells

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Acknowledgements

We thank Dr. Y. Tang (Department of Histology and Embryology, Chongqing University of Medical Sciences) for critical reading the manuscript. This work was supported in parts by grants no. 30470911 and 30570983 from the National Science Foundation of China (NSFC). Feng Mei and Bin Yu contributed equally to this work.

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Authors and Affiliations

  1. Department of Histology and Embryology, Third Military Medical University, Chongqing, 400038, People’s Republic of China

    Feng Mei, Bin Yu, Hua Ma, Hong-jun Zhang & De-shan Zhou

  2. Department of Histology and Embryology, Capital University of Medical Sciences, Beijing, 100054, People’s Republic of China

    De-shan Zhou

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  1. Feng Mei
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  2. Bin Yu
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  3. Hua Ma
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Correspondence to De-shan Zhou.

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Mei, F., Yu, B., Ma, H. et al. Interstitial cells of Cajal could regenerate and restore their normal distribution after disrupted by intestinal transection and anastomosis in the adult guinea pigs. Virchows Arch 449, 348–357 (2006). https://doi.org/10.1007/s00428-006-0258-6

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