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Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis

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Abstract

Abnormalities in humoral immunity are implicated in the pathogenesis of ulcerative colitis. However, the detailed mechanisms of B-cell activation in the locale remain unaccounted for. We analyzed ulcerative colitis from the standpoint of lymphocytic expansion in the loco. Intestinal specimens obtained at surgery from 30 patients with ulcerative colitis treated with corticosteroids and 15 with Crohn’s disease were analyzed by immunohistochemistry and flow cytometry. Ulcerative colitis was characterized by a diffuse distribution of Ki-67+ small round cells particularly in the ulcer base (that were CD19+ and CD20–), with a significant number of them also CD138+. Immunoelectron microscopy for CD19 revealed an abundance of rough endoplasmic reticulum in the cytoplasm. These indicated that they are of immature plasma lineage cells. By contrast, plasma cells in Crohn’s disease were negative for CD19, and the labeling for Ki-67 was infrequent, showing mature phenotype. Flow cytometry revealed an occurrence of CD19+ and CD20– cells in ulcerative colitis but not in Crohn’s disease. The labeling index of Ki-67 among CD19+ plasma cells was positively correlated with the clinical activity of ulcerative colitis. High labeling of Ki-67 in CD19+ plasma cells is specific for active ulcerative colitis that was resistant to medical treatment by corticosteroids.

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Acknowledgements

The authors wish to thank Dr. Atsuo Kitano, Dr. Yukio Nishiguchi, Yoshitaka Kinouchi, and Iwao Sasaki for helpful discussions. This work was supported in part by grants-in-aid for cancer research and a grant for intractable inflammatory bowel disease from the Ministry of Health, Labor, and Welfare, Japan.

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Authors and Affiliations

  1. Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka, Japan

    Yoshio Jinno, Shiro Nakamura, Nobuhide Oshitani, Takayuki Matsumoto & Tetsuo Arakawa

  2. Department of Pathology, National Hospital Organization Mito Medical Center, Ibaraki, Japan

    Haruo Ohtani

  3. Department of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan

    Motoji Oki

  4. Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan

    Kiyoshi Maeda

  5. Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan

    Kohei Fukushima

  6. Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan

    Haruo Ohtani & Hiroshi Nagura

  7. Department of Internal Medicine, Division of Gastroenterology, Hyogo College of Medicine, Nishinomiya, Japan

    Yoshio Jinno & Takayuki Matsumoto

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  1. Yoshio Jinno
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  2. Haruo Ohtani
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  3. Shiro Nakamura
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  4. Motoji Oki
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  5. Kiyoshi Maeda
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  6. Kohei Fukushima
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  7. Hiroshi Nagura
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  8. Nobuhide Oshitani
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  9. Takayuki Matsumoto
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  10. Tetsuo Arakawa
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Correspondence to Yoshio Jinno.

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Jinno, Y., Ohtani, H., Nakamura, S. et al. Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis. Virchows Arch 448, 412–421 (2006). https://doi.org/10.1007/s00428-005-0136-7

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  • DOI: https://doi.org/10.1007/s00428-005-0136-7

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