Abstract
Leaf tissues of barley (Hordeum vulgare L. cv. Salome) respond to methyl jasmonate (JaMe) treatment with a characteristic pattern of gene expression. Jasmonate-induced proteins (JIPs), such as leaf thionins (jip15 gene product) and ribosome-inactivating proteins (jip60 gene product), rapidly accumulate. Their genes are transiently transcriptionally activated, as shown here by the determination of in-vitro transcription rates in run-off assays. In contrast to jip genes, expression of photosynthetic genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS gene product) and a type III light-harvesting chlorophyll-a/b-binding protein (LHCP; lhbC1 gene product), for example, was rapidly down-regulated in JaMe-treated barley leaves. Despite decreasing rates of rbcS and lhbC1 gene transcription, their transcripts were maintained in JaMe-treated leaf tissues for at least 36 h. Only at a later stage, was there a decline in the levels of rbcS and lhbC1, but not jip, transcripts, suggesting a selective destabilization of photosynthetic mRNAs in JaMe-treated leaf tissues.
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Abbreviations
- JaMe:
-
jasmonic acid methyl ester (methyl jasmonate)
- JIP(s):
-
jasmonate-induced protein(s)
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
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Dedicated to Professor Dr. Andreas Sievers, Bonn, on the occasion of his 65th birthday
This work was supported in part by a research project grant (to B.P.) from the Stiftung Volkswagenwerk, Hannover, Germany. We are grateful to Dr. J. Henstrand (Institut for Plant Sciences, Zürich, Switzerland) for critical reading of the manuscript. We wish to thank Drs. J. Lehmann (Institut for Plant Biochemistry, Halle/ Saale, Germany) for providing us with antibodies against JIP60, and W. Becker (Rheinisch-Westfälische Technische Hochschule, Aachen, Germany), J. Brandt (The Royal Veterinary and Agricultural University, Copenhagen, Denmark), R.B. Meagher (The University of Georgia, Athens, Georgia, USA), and R. Cerff (Institute for Genetics, Braunschweig, Germany) for the different cDNA clones used in this study.
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Reinbothe, C., Parthier, B. & Reinbothe, S. Temporal pattern of jasmonate-induced alterations in gene expression of barley leaves. Planta 201, 281–287 (1997). https://doi.org/10.1007/s004250050067
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DOI: https://doi.org/10.1007/s004250050067