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Cytokinin affects nuclear- and plastome-encoded energy-converting plastid enzymes

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Abstract

Cytokinins induce two specific morphological alterations in mosses: (i) the differentiation of a tipgrowing cell into a three-faced apical cell (the so-called bud), and (ii) the division of chloroplasts. In a developmental mutant of the moss Physcomitrella patens (Hedw.) B.S.G. (mutant PC22) impeded in both cellular differentiation (bud production) and chloroplast division, addition of cytokinin (N6-Δ2-isopentenyladenine) led to bud production after 3 d in the wild type and after 7 d in the mutant. Hormone induced a division of the mutant macrochloroplasts starting within 24 h and ongoing for 72 h. During this period the abundances of several plastid proteins changed in both genotypes as judged by two-dimensional-protein gel electrophoresis, silver staining and subsequent quantification with novel computer software. Eight of these polypeptides were isolated independently, subjected to microsequencing and thus identified, resulting in the first protein sequence data from a moss. Three polypeptides (24 kDa, 22 kDa, 20 kDa) were found to be homologous to enhancer protein OEE2 of the oxygen-evolving complex, four to represent isoforms of phosphoglycerate kinase (EC 2.7.2.3), and one was identified as the β-chain of chloroplast ATPase (EC 3.6.1.34). Possible involvement of these key enzymes of the chloroplast energy-conversion machinery in organelle division and in cellular differentiation is discussed. Further sequence information was obtained from both subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39). Amounts of these polypeptides were not appreciably affected by cytokinin in moss chloroplasts.

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Abbreviations

IEF:

isoelectric focusing

LHC:

light-harvesting complex

PGK:

phosphoglycerate kinase

OEE2:

enhancer protein 2 of the oxygen-evolving complex

pI:

isoelectric point

Rubisco:

ribulose-1,5-bisphosphate carboxylase/oxygenase

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, Ohnhorststrasse 18, D-22609, Hamburg, Germany

    Britta Kasten & Ralf Reski

  2. Institut für Zellbiochemie und klinische Neurobiologie, Universitätskrankenhaus Eppendorf, Süderfeldstrasse 24, D-22529, Hamburg, Germany

    Friedrich Buck

  3. Biometra, Rudolf-Wissell-Strasse 30, D-37079, Göttingen, Germany

    Joachim Nuske

Authors
  1. Britta Kasten
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  2. Friedrich Buck
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  3. Joachim Nuske
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  4. Ralf Reski
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Corresponding author

Correspondence to Ralf Reski.

Additional information

This article is dedicated to Professor Wolfgang O. Abel on the occasion of his 65th birthday

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Kasten, B., Buck, F., Nuske, J. et al. Cytokinin affects nuclear- and plastome-encoded energy-converting plastid enzymes. Planta 201, 261–272 (1997). https://doi.org/10.1007/s004250050065

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  • DOI: https://doi.org/10.1007/s004250050065

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