Abstract
Main conclusion
In Medicago sativa nodulated roots, NR-dependent NO production is involved in maintaining energy state, presumably through phytoglobin NO respiration, under both salinity and hypoxia stress.
Abstract
The response to low and average salinity stress and to a 5 day-long flooding period was analyzed in M. sativa nodulated roots. The two treatments result in a decrease in the biological nitrogen fixation capacity and the energy state (evaluated by the ATP/ADP ratio), and conversely in an increase nitric oxide (NO) production. Under salinity and hypoxia treatments, the use of either sodium tungstate, an inhibitor of nitrate reductase (NR), or carboxy-PTIO, a NO scavenger, results in a decrease in NO production and ATP/ADP ratio, meaning that NR-dependent NO production participates to the maintenance of the nodulated roots energy state.
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Abbreviations
- ARA:
-
Acetylene reducing activity
- BNF:
-
Biological nitrogen fixation
- cPTIO:
-
2-[4-carboxyphenyl]-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
- DAF-2:
-
4,5-diaminofluorescein
- NR:
-
Nitrate reductase
- Phytogb:
-
Phytoglobin
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Acknowledgements
This work was supported by INRAE and by the Comité Mixte Franco-Tunisien pour la Coopération Universitaire (PHC Utique, Grant no. 17G0904).
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Aridhi, F., Sghaier, H., Gaitanaros, A. et al. Nitric oxide production is involved in maintaining energy state in Alfalfa (Medicago sativa L.) nodulated roots under both salinity and flooding. Planta 252, 22 (2020). https://doi.org/10.1007/s00425-020-03422-1
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DOI: https://doi.org/10.1007/s00425-020-03422-1