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Raman spectroscopy as an early detection tool for rose rosette infection

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Abstract

Main conclusion

Hand-held Raman spectroscopy is a potential tool for a confirmatory, non-invasive, and non-destructive detection and identification of rose rosette disease. Using this spectroscopic approach, structural changes in roses that are associated with this viral infection can be revealed.

Abstract

The commercial rose shrub industry in the United States is one of the largest of its kind. All commercial rose varieties are susceptible to rose rosette disease (RRD), a deadly viral disease vectored by eriophyid mites. This disease is typically diagnosed visually and/or by PCR-based detection assays. The present work demonstrates that Raman spectroscopy can detect RRD in intact leaf tissue. It is shown that chemometric analysis can distinguish between spectra collected from symptomatic and asymptomatic tissue, as well as between healthy and asymptomatic tissue. This method will be useful as an initial screen for RRD prior to PCR analysis to help conserve reagents and save time.

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Acknowledgements

The authors are grateful for the financial support of Agrilife Research at Texas A&M and acknowledge the Governor’s University Research Initiative (GURI) grant program of Texas A&M, GURI Grant Agreement No. 12-2016, M1700437.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA

    Charles Farber & Dmitry Kurouski

  2. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843, USA

    Madalyn Shires & Kevin Ong

  3. Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843, USA

    David Byrne

  4. The Institute for Quantum Science and Engineering, Texas A&M University, College Station, TX, 77843, USA

    Dmitry Kurouski

Authors
  1. Charles Farber
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  2. Madalyn Shires
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  3. Kevin Ong
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  4. David Byrne
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  5. Dmitry Kurouski
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Correspondence to Dmitry Kurouski.

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Farber, C., Shires, M., Ong, K. et al. Raman spectroscopy as an early detection tool for rose rosette infection. Planta 250, 1247–1254 (2019). https://doi.org/10.1007/s00425-019-03216-0

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  • DOI: https://doi.org/10.1007/s00425-019-03216-0

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