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Analysis of genetic composition and transmitted parental heterozygosity of natural 2n gametes in Populus tomentosa based on SSR markers

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Abstract

Main conclusion

Natural 2n female gametes and transmission of parental heterozygosity by natural 2n gametes in Populus tomentosa are reported for the first time, which provides a new approach to polyploid breeding.

Abstract

Naturally occurring 2n pollen is widespread in Populus tomentosa and plays an important role in polyploid breeding. However, the competitiveness of 2n pollen is lower than that of haploid pollen during pollination and fertilization, so 2n pollen is less efficient at fertilizing haploid female gametes to produce polyploids. In theory, polyploids can also be obtained when 2n female gametes are fertilized by haploid pollen. Thus, the question becomes whether natural 2n female gametes exist in P. tomentosa, which can be answered by examining the genetic composition of natural 2n gametes. In this study, the origin of 87 triploids from the hybrid combination “X-2 × Z-5” was identified by SSR markers and 21% of natural 2n gametes were found to originate from female parents. Four SSR loci with low recombination rates were used to identify the genetic composition of natural 2n gametes. The results showed that the genetic composition of 2n female gametes was mainly characterized by SDR, while 2n male gametes were mainly produced by FDR. Moreover, the transmission of parental heterozygosity by natural 2n gametes, which is significantly different between female and male parents in FDR and SDR types, was analysed using 42 SSR primers. Here, we report naturally occurring 2n female gametes for the first time in P. tomentosa and reveal the genetic constitution and transmitted parental heterozygosity of these gametes. Our results provide a foundation for theoretical research into 2n gametes and their application in new polyploid breeding strategies.

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Abbreviations

SDR:

Second division restitution

FDR:

First division restitution

hDNA:

Heteroduplex DNA

K–S:

Kolmogorov–Smirnov

HR:

Homologous recombination

CO:

Crossing-over

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Acknowledgements

The authors would like to thank the staff of Guanxian nursery in Shandong Province, China for providing the experimental field. We are also grateful to the anonymous reviewers and editors for their constructive comments. This work was supported by National Natural Science Foundation of China (31530012).

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Authors and Affiliations

  1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, 100083, China

    Zhiqiang Han & Xiangyang Kang

  2. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China

    Zhiqiang Han, Xining Geng, Kang Du, Congping Xu, Pengqiang Yao, Fengying Bai & Xiangyang Kang

  3. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Zhiqiang Han, Xining Geng, Kang Du, Congping Xu, Pengqiang Yao, Fengying Bai & Xiangyang Kang

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  1. Zhiqiang Han
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  2. Xining Geng
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Correspondence to Xiangyang Kang.

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Han, Z., Geng, X., Du, K. et al. Analysis of genetic composition and transmitted parental heterozygosity of natural 2n gametes in Populus tomentosa based on SSR markers. Planta 247, 1407–1421 (2018). https://doi.org/10.1007/s00425-018-2871-4

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  • DOI: https://doi.org/10.1007/s00425-018-2871-4

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