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An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development

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Abstract

Main conclusion

In this study, we reported that a F-box protein, OsADF, as one of the direct targets of TDR , plays a critical role in rice tapetum cell development and pollen formation.

Abstract

The tapetum, the innermost sporophytic tissue of anther, plays an important supportive role in male reproduction in flowering plants. After meiosis, tapetal cells undergo programmed cell death (PCD) and provide nutrients for pollen development. Previously we showed that tapetum degeneration retardation (TDR), a basic helix-loop-helix transcription factor, can trigger tapetal PCD and control pollen wall development during anther development. However, the comprehensive regulatory network of TDR remains to be investigated. In this study, we cloned and characterized a panicle-specific expression F-box protein, anther development F-box (OsADF). By qRT-PCR and RNA in situ hybridization, we further confirmed that OsADF expressed specially in tapetal cells from stage 9 to stage 12 during anther development. In consistent with this specific expression pattern, the RNAi transgenic lines of OsADF exhibited abnormal tapetal degeneration and aborted microspores development, which eventually grew pollens with reduced fertility. Furthermore, we demonstrated that the TDR, a key regulator in controlling rice anther development, could regulate directly the expression of OsADF by binding to E-box motifs of its promoter. Therefore, this work highlighted the possible regulatory role of TDR, which regulates tapetal cell development and pollen formation via triggering the possible ADF-mediated proteolysis pathway.

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Acknowledgments

We thank Zong Jie for data analysis, Yi Wenwei for rice transformation, Luo Qingsong for field work. Prof. Zhang Dabing and Prof. Yuan Zheng from Shanghai Jiao Tong University is gratefully acknowledged for his valuable suggestions on the experimental design and manuscript. This study was supported by the National Natural Science Foundation of China (#31201184), Natural Science Foundation of Hunan Province, China (#14JJ2138), the National Key Programs for Transgenic Crops (#2011ZX08001-004) and the Program of Breeding and Application of hybrid Rice with Strong Heterosis (#2011AA10A101).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, Changsha, 410125, China

    Li Li, Shufeng Song, Huafeng Deng, Na Li, Xiqin Fu & Longping Yuan

  2. Collaborative Innovation Center for Modern Production of Multiple Cropping System in Southern Paddy Area, Changsha, 410128, China

    Li Li, Huafeng Deng & Guanghui Chen

  3. Hunan Agricultural University, Changsha, 410128, China

    Yixing Li & Guanghui Chen

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  1. Li Li
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Correspondence to Longping Yuan.

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Li, L., Li, Y., Song, S. et al. An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development. Planta 241, 157–166 (2015). https://doi.org/10.1007/s00425-014-2160-9

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