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Recovery from water stress affects grape leaf petiole transcriptome

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Abstract

Fast and efficient recovery from water stress is a key determinant of plant adaptation to changing meteorological conditions modulating transpiration, i.e. air temperature and humidity. We analysed transcriptomic responses during rehydration after water stress in grapevine leaf petioles, where embolism formation and repair commonly take place, and where metabolic changes related to embolism recovery are expected to be particularly important. We compared gene expression of recovering plants with irrigated controls, upon high and low transpiration conditions, using cDNA microarrays. In parallel, we assessed the daily dynamics of water relations, embolism formation and repair, and leaf abscisic acid concentration. In recovering plants, the most affected gene categories were secondary metabolism, including genes linked to flavonoid biosynthesis; sugar metabolism and transport, and several aquaporin genes. The physiological dynamics of recovery were lower and the number of differentially expressed probes was much lower upon low transpiration than found in actively transpiring grapevines, suggesting the existence of a more intense metabolic reorganization upon high transpiration conditions and of a signal eliciting these responses. In plants recovering under high transpiration, abscisic acid concentrations significantly increased, and, in parallel, transcripts linked to abscisic acid metabolism and signalling (ABA-8′-hydroxylase, serine-threonine kinases, RD22 proteins) were upregulated; a trend that was not observed upon low transpiration. Our results show that recovery from water stress elicits complex transcriptomic responses in grapevine. The increase observed in abscisic acid cellular levels could represent a signal triggering the activation of responses to rehydration after stress.

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Abbreviations

ABA:

Abscisic acid

IRR:

Irrigated

WS:

Water stress

REC:

Recovery

HCFM:

Hydraulic conductance flow meter

qRT-PCR:

Quantitative real-time PCR

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Acknowledgments

The authors wish to thank Dr. Claudio Bonghi from the University of Padova, for assistance in designing the microarray protocols, Vivai Cooperativi Rauscedo—Italy, for providing grapevines, and two anonymous reviewers for having improved former versions of this manuscript.

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Authors and Affiliations

  1. Dipartimento di Colture arboree, University of Torino, Via Leonardo da Vinci 44, 10095, Grugliasco (TO), Italy

    Irene Perrone, Chiara Pagliarani, Claudio Lovisolo, Walter Chitarra, Federica Roman & Andrea Schubert

  2. Plant Virology Institute, National Research Council (IVV-CNR), Grugliasco Unit, Via Leonardo da Vinci 44, 10095, Grugliasco (TO), Italy

    Claudio Lovisolo

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  1. Irene Perrone
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  2. Chiara Pagliarani
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  3. Claudio Lovisolo
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  4. Walter Chitarra
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  5. Federica Roman
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  6. Andrea Schubert
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Correspondence to Claudio Lovisolo.

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I. Perrone and C. Pagliarani equally contributed to this paper.

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Perrone, I., Pagliarani, C., Lovisolo, C. et al. Recovery from water stress affects grape leaf petiole transcriptome. Planta 235, 1383–1396 (2012). https://doi.org/10.1007/s00425-011-1581-y

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