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The chloroplast outer membrane protein CHUP1 interacts with actin and profilin

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Abstract

Chloroplasts accumulate in response to low light, whereas high light induces an actin-dependent avoidance movement. This is a long known process, but its molecular base is barely understood. Only recently first components of the blue light perceiving signal cascade initiating this process were described. Among these, a protein was identified by the analysis of a deletion mutant in the corresponding gene resulting in a chloroplast unusual positioning phenotype. The protein was termed CHUP1 and initial results suggested chloroplast localization. We demonstrate that the protein is indeed exclusively and directly targeted to the chloroplast surface. The analysis of the deletion mutant of CHUP1 using microarray analysis shows an influence on the expression of genes found to be up-regulated, but not on genes found to be down-regulated upon high light exposure in wild-type. Analyzing a putative role of CHUP1 as a linker between chloroplasts and the cytoskeleton, we demonstrate an interaction with actin, which is independent on the filamentation status of actin. Moreover, binding of CHUP1 to profilin—an actin modifying protein—could be shown and an enhancing effect of CHUP1 on the interaction of profilin to actin is demonstrated. Therefore, a role of CHUP1 in bridging chloroplasts to actin filaments and a regulatory function in actin polymerization can be discussed.

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Abbreviations

Toc:

Translocon at the outer chloroplastic envelope membrane

PLP:

Poly-L-proline

PRM1:

Profilin-binding motif 1

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Acknowledgements

We specially thank Prof. Chris Hawes from Oxford Brookes University for providing us with the Golgi marker ST-GFP (rat sialyl transferase fused to GFP, Boevink et al. 1998). This work was supported by grant to E.S. from the Volkswagenstiftung.

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Authors and Affiliations

  1. Department of Biology I, LMU München, Menzinger Str. 67, 80638, Munich, Germany

    Serena Schmidt von Braun & Enrico Schleiff

  2. Department of Biosciences, JWGU Frankfurt am Main, Cluster of Excellence Macromolecular Complexes, Max-von-Laue Str. 9, 60438, Frankfurt, Germany

    Enrico Schleiff

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  1. Serena Schmidt von Braun
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  2. Enrico Schleiff
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Correspondence to Enrico Schleiff.

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Schmidt von Braun, S., Schleiff, E. The chloroplast outer membrane protein CHUP1 interacts with actin and profilin. Planta 227, 1151–1159 (2008). https://doi.org/10.1007/s00425-007-0688-7

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