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Identification of genes responsive to brown planthopper Nilaparvata lugens Stål (Homoptera: Delphacidae) feeding in rice

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Abstract

The brown planthopper (Nilaparvata lugens Stål) is a rice (Oryza sativa L.)-specific herbivore that ingests assimilates from the phloem, which leads to stunted growth and therefore poor yield of rice. In this study, 27 planthopper-responsive genes in rice plants were isolated by suppression subtractive hybridization (SSH), and confirmed by filter cDNA array hybridization and northern blot analysis. Among these transcripts, 25 were induced and 2 were suppressed by N. lugens feeding. The responsive genes are involved in photosynthesis, macromolecule degradation, signal transduction and defenses against stress and pathogens. Overall, genes involved in macromolecule degradation and plant defenses were up-regulated, whereas those involved in photosynthesis and cell growth were down-regulated. Our data suggest that leaf senescence is most likely activated in the susceptible rice plants damaged by N. lugens insects.

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Abbreviations

EST :

Expressed sequence tag

IMPDH :

Inosine 5′-monophosphate dehydrogenase

JA :

Jasmonic acid

LHC :

Light-harvesting complex

SA :

Salicylic acid

SSH :

Suppression subtractive hybridization

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Acknowledgements

This research was supported by grants from the National Special Key Project on Functional Genomics and Biochip of China and the National Natural Science Foundation of China (30170085).

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, People’s Republic of China

    Hongyu Yuan, Xinping Chen, Lili Zhu & Guangcun He

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  1. Hongyu Yuan
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  2. Xinping Chen
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  3. Lili Zhu
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  4. Guangcun He
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Correspondence to Guangcun He.

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Yuan, H., Chen, X., Zhu, L. et al. Identification of genes responsive to brown planthopper Nilaparvata lugens Stål (Homoptera: Delphacidae) feeding in rice. Planta 221, 105–112 (2005). https://doi.org/10.1007/s00425-004-1422-3

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  • DOI: https://doi.org/10.1007/s00425-004-1422-3

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