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Neuronal expression of the intermediate conductance calcium-activated potassium channel KCa3.1 in the mammalian central nervous system

  • Ion channels, receptors and transporters
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Abstract

The expression pattern and functional roles for calcium-activated potassium channels of the KCa2.x family and KCa1.1 have been extensively examined in central neurons. Recent work indicates that intermediate conductance calcium-activated potassium channels (KCa3.1) are also expressed in central neurons of the cerebellum and spinal cord. The current study used immunocytochemistry and GFP linked to KCNN4 promoter activity in a transgenic mouse to determine the expression pattern of KCa3.1 channels in rat or mouse neocortex, hippocampus, thalamus, and cerebellum. KCa3.1 immunolabel and GFP expression were closely matched and detected in both excitatory and inhibitory cells of all regions examined. KCa3.1 immunolabel was localized primarily to the somatic region of excitatory cells in cortical structures but at the soma and over longer segments of dendrites of cells in deep cerebellar nuclei. More extensive labeling was apparent for inhibitory cells at the somatic and dendritic level with no detectable label associated with axon tracts or regions of intense synaptic innervation. The data indicate that KCa3.1 channels are expressed in the CNS with a differential pattern of distribution between cells, suggesting important functional roles for these calcium-activated potassium channels in regulating the excitability of central neurons.

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Acknowledgments

We gratefully acknowledge L. Chen for expert technical assistance, the generous preparation and donation of fixed GAD67-GFP knock-in mouse brains by J-C. Lacaille and I. Laplante (Université de Montréal, Canada) originally supplied by Y. Yanagawa (Gunma University, Japan), and D.E. Rancourt and F.R. Jirik for advice in the design of a BAC construct. This work was supported by operating grants from the Canadian Institute of Health Research (R.W.T., G.W.Z.) and a studentship through Alberta Innovates—Health Solutions (AI-HS) (M.T.). R.W.T. and G.W.Z. are AI-HS Scientists and G.W.Z. holds a Canada Research Chair.

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  1. Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3330 Hospital Dr. N.W., Calgary, Alberta, Canada, T2N 4N1

    Ray W. Turner, Mirna Kruskic & Michelle Teves

  2. Department of Physiology and Pharmacology, Hotchkiss Brain Institute, University of Calgary, 3330 Hospital Dr. N.W., Calgary, Alberta, Canada, T2N 4N1

    Ray W. Turner, Shahid Hameed & Gerald W. Zamponi

  3. Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada, T2N 4N1

    Teresa Scheidl-Yee

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Turner, R.W., Kruskic, M., Teves, M. et al. Neuronal expression of the intermediate conductance calcium-activated potassium channel KCa3.1 in the mammalian central nervous system. Pflugers Arch - Eur J Physiol 467, 311–328 (2015). https://doi.org/10.1007/s00424-014-1523-1

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