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Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection

  • Integrative Physiology
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Abstract

Acute rejection in renal transplantation disturbs solute and volume maintenance in humans accompanied by delayed graft function and poor prognosis. We recently reported that decreased expression and function of Na+/H+ exchanger type 3 (NHE-3) in proximal tubules and epithelial Na+ channels and aquaporin 2 in collecting ducts are major mechanisms involved in Na+ and water imbalances shortly after transplantation in rat undergoing acute rejection. We performed kidney transplantations in rats with bilaterally nephrectomized recipients with acute rejection and, in addition, systemically administered a specific inhibitor of NHE-3 (NHE-I). NHE inhibition in acute renal failure was shown to improve tubular function and recovery. The aim of this therapy was to reduce energy consumption of the graft and preserve NHE-3 function. Imbalances in electrolyte excretion declined in NHE-I-treated animals and NHE-3 activity was preserved. Observed NHE-I-dependent changes in electrolyte excretion, polyuria, and reduced protein reabsorption in the acute postoperative phase are predictors of favorable graft outcome in humans.

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Acknowledgment

The excellent technical help from Mrs. Ute Neugebauer is gratefully acknowledged. This study was supported by a grant from the Else-Kröner-Fresenius Foundation (P22/05//A43/05//F00) to E.S.

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Authors and Affiliations

  1. Medizinische Klinik und Poliklinik D, Experimentelle Nephrologie, Universitätsklinikum Münster, Münster, Germany

    Stefan Reuter, Ana Velic, Bayram Edemir, Rita Schröter, Hermann Pavenstädt, Gert Gabriëls & Eberhard Schlatter

  2. Physiologisches Institut, Universität Kiel, Kiel, Germany

    Markus Bleich

Authors
  1. Stefan Reuter
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  2. Ana Velic
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  3. Bayram Edemir
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  4. Rita Schröter
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  5. Hermann Pavenstädt
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  6. Gert Gabriëls
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  7. Markus Bleich
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  8. Eberhard Schlatter
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Corresponding author

Correspondence to Eberhard Schlatter.

Additional information

Stefan Reuter and Ana Velic contributed equally to this study.

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Reuter, S., Velic, A., Edemir, B. et al. Protective role of NHE-3 inhibition in rat renal transplantation undergoing acute rejection. Pflugers Arch - Eur J Physiol 456, 1075–1084 (2008). https://doi.org/10.1007/s00424-008-0484-7

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  • DOI: https://doi.org/10.1007/s00424-008-0484-7

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