Abstract
Purpose
Delayed-onset muscle soreness (DOMS) describes an entity characterized by ultrastructural muscle damage. Hesperidin methyl chalcone (HMC) is a synthetic flavonoid presenting analgesic, anti-inflammatory, and antioxidant properties. We evaluated the effects of HMC upon DOMS.
Method
In a preventive paradigm, 31 sedentary young men were submitted to a randomized, double-blinded parallel trial and received HMC 500 mg or one placebo capsule × 3 days before an intense dynamic exercise protocol (concentric/eccentric actions) applied for lower limbs for inducing muscle damage. Assessments were conducted at baseline, and 24 and 48 h after, comprising physical performance, and post-muscle soreness and damage, inflammation, recovery of muscle strength, and postural balance associated with DOMS. HMC safety was also evaluated. Thirty participants completed the study.
Results
HMC improved the performance of participants during exercise (40.3 vs 51.3 repetitions to failure, p = 0.0187) and inhibited CPK levels (90.5 vs 57.9 U/L, p = 0.0391) and muscle soreness during passive quadriceps palpation (2.6 vs 1.4 VAS cm, p = 0.0439), but not during active actions, nor did it inhibit IL-1β or IL-10 levels. HMC improved muscle strength recovery, and satisfactorily refined postural balance, without inducing injury to kidneys or liver.
Conclusions
Preemptive HMC supplementation may be beneficial for boosting physical performance and for the amelioration of clinical parameters related to DOMS, including pain on muscle palpation, increased blood CPK levels, and muscle strength and proprioceptive deficits, without causing adverse effects. These data advance the understanding of the benefits provided by HMC for DOMS treatment, which supports its usefulness for such purpose.
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Data availability
The data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Abbreviations
- 1RM:
-
One-maximum repetition
- ANOVA:
-
Analysis of variance
- AP:
-
Anteroposterior
- ATP:
-
Adenosine triphosphate
- BMI:
-
Body mass index
- COP:
-
Center-of-pressure
- CPK:
-
Creatine phosphokinase
- DOMS:
-
Delayed-onset muscle soreness
- EDTA:
-
Ethylenediaminetetraacetic acid
- IDEP:
-
Intense dynamic exercise protocol
- EF:
-
Effect size
- ELISA:
-
Enzyme-linked immunosorbent assay
- EIMD:
-
Exercise-induced muscle damage
- GGT:
-
Gamma glutamyl transferase
- HMC:
-
Hesperidin methyl chalcone
- IL-1β:
-
Interleukin-1β
- IL-10:
-
Interleukin-10
- ML:
-
Mediolateral
- NFκB :
-
Nuclear factor κB
- NSAIDs:
-
Non-steroidal anti-inflammatory drugs
- PLA:
-
Placebo
- WHO:
-
World Health Organization
- VAS:
-
Visual analogue scale
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This work was supported by grants from Fundação Nacional de Desenvolvimento do Ensino Superior Particular (FUNADESP; Grant No. 5301159) research fellowship and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) senior research fellowship (309633/2021-4). THZ and TSS received CAPES PhD bursary (finance code 001).
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MZL, AFA, AKSA, THZ, OKH, TSS, MMB, NAS, EOJ, WAVJ, and SMB conducted data acquisition, analysis, and interpretations. AFA, OKH, EOJ, and WAVJ contributed with reagents, analytical tools, and expert intellectual support for the study. SMB conceived and designed the study and supervised the project. MZL, WAVJ, and SMB wrote and critically revised the manuscript. All authors contributed to the content, critical review, and approved the final version of the manuscript.
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Luque, M.Z., Aguiar, A.F., da Silva-Araújo, A.K. et al. Evaluation of a preemptive intervention regimen with hesperidin methyl chalcone in delayed-onset muscle soreness in young adults: a randomized, double-blinded, and placebo-controlled trial study. Eur J Appl Physiol 123, 1949–1964 (2023). https://doi.org/10.1007/s00421-023-05207-2
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DOI: https://doi.org/10.1007/s00421-023-05207-2