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Ramp vs. step tests: valid alternatives to determine the maximal lactate steady-state intensity?

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European Journal of Applied Physiology Aims and scope Submit manuscript

Abstract

Purpose

The aims of this study were (1) to investigate if the respiratory compensation point (RCP) as derived from ramp incremental (RI) exercise could accurately predict the power output (PO) at the maximal lactate steady state (MLSS), and (2) to compare its accuracy with the second lactate threshold (LT2) obtained from step incremental (SI) exercise.

Methods

Nineteen participants performed a RI test (30 W·min−1) to determine RCP, a SI test (30 or 40 W·3 min−1) to determine LT2, and two or more constant work rate (CWR) tests to determine MLSS. For each participant, the \(\dot{\text{V}}\)O2/PO relationship for RI and CWR exercise was established. The ramp-identified PO at RCP was corrected by accounting for the gap between these relationships using the individually determined \(\dot{\text{V}}\) O2/PO regression above GET (RCPcorr-1) or using a fixed regression slope (RCPcorr-2). LT2 was determined using four methods: Dmax, modified Dmax (ModDmax), 4-mM threshold (LT4mM) and an expert-determined LT2 (LT2-expert).

Results

RCPcorr-1 (235 ± 69 W), RCPcorr-2 (228 ± 58 W) and LT2-expert (227 ± 61 W) were not different from MLSS (225 ± 60 W). Dmax (203 ± 53 W) underestimated MLSS, while RCP (280 ± 60 W), ModDmax (235 ± 67 W) and LT4mM (234 ± 68 W) overestimated MLSS. The \(\dot{\text{V}}\)O2 at RCP (3.13 ± 0.79L·min−1) and LT2-expert (2.99 ± 0.19L·min−1) did not differ from MLSS (3.05 ± 0.72 L·min−1).

Conclusion

This study demonstrated that RCP as derived from RI exercise and LT2 as derived from SI exercise can be equally accurate to determine the PO associated with MLSS. Although these results confirmed the suitability of RI and SI tests for this purpose, they also highlighted the importance of an appropriate threshold method selection and the eye of the expert.

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Abbreviations

ANOVA:

Analysis of variance

CWR:

Constant work rate

GET:

Gas exchange threshold

HR:

Heart rate

ICC:

Intraclass correlation coefficient

La- :

Lactate

LT2 :

Second lactate threshold

LoA:

Limits of agreement

MLSS:

Maximal lactate steady state

MMSS:

Maximal metabolic steady state

MRT:

Mean response time

PO:

Power output

RI:

Ramp incremental

RCP:

Respiratory compensation point

SI:

Step incremental

CI95% :

95% confidence interval

References

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Acknowledgments

The authors would like to thank the subjects for their commitment to the study. This study was funded by the Special Research Fund of the Ghent University (Ghent, Belgium).

Funding

This research was funded by the Special Research Fund of the Ghent University (Ghent, Belgium) in the context of a doctoral fellowship (n° BOFDOC 2016003101).

Author information

Authors and Affiliations

Authors

Contributions

KC, SP, JGB and JB conceived and designed research. KC, ML and KV conducted the experiments and analyzed the data. KC, SP and JB wrote the manuscript. All authors revised and approved the manuscript.

Corresponding author

Correspondence to Jan Boone.

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Conflicts of interest

Results of the present study are presented clearly, honestly and without fabrication, falsification or inappropriate data manipulation. No conflicts of interest, financial or otherwise, are declared by the authors.

Ethics approval

This study was approved by the Ethical Committee of the Ghent University Hospital (Ghent, Belgium).

Consent to participate

Informed consent for participation in this study was received from all subjects.

Consent for publication

Consent for publication of the current study results was received from all subjects.

Availability of data and material

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Additional information

Communicated by Jean -Rene Lacour.

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Caen, K., Pogliaghi, S., Lievens, M. et al. Ramp vs. step tests: valid alternatives to determine the maximal lactate steady-state intensity?. Eur J Appl Physiol 121, 1899–1907 (2021). https://doi.org/10.1007/s00421-021-04620-9

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  • DOI: https://doi.org/10.1007/s00421-021-04620-9

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