Abstract
Mott cells are plasma cells that have multiple spherical Russell bodies packed in their cytoplasm. Russell bodies are dilated endoplasmic reticulum cisternae filled with aggregates of immunoglobulins that are neither secreted nor degraded. Mott cells were observed in our study by light and electron microscope in the lymph nodes of rats with experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Mott cells were detected on hematoxylin and eosin (HE)-stained lymph node sections as vacuolated cells with eccentrically positioned nuclei and large number of faint blue spherical inclusions in the cytoplasm. Electron microscopic investigation revealed the presence of Russell bodies of the “medusa” form inside Mott cells in lymph node ultra-thin sections of EAE animals. Mott cells expressed the plasma cell marker CD138 and either kappa or lambda immunoglobulin light chains, indicating their origin from polyclonally activated B cells. Finally, Mott cells were associated with active EAE, as they were not found in the lymph nodes of EAE-resistant Albino Oxford rats. The presence of Russell bodies implies an excessive production of immunoglobulins in EAE, thus further emphasizing the role of B cells, and among them Mott cells, in the pathogenesis of this animal model of multiple sclerosis.
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The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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This work was partly supported by the Ministry of Science of the Republic of Serbia (grant numbers 41025 and 175090) and Serbian Academy of Sciences and Arts (grant number F-35).
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Each author’s contribution to the paper is quantified as following: TM, SV, and DC: were responsible for acquisition of data. DC, TM, VCM, and TKS: analyzed data. SV, JT, ZS, and SP: performed experiments on animals. TM, ND, and DC: processed the samples for light and electron microscopy. DC, TM, TKS, AI, VT, and VB: designed the study. TKS, VT, DC, SV, and TM: wrote the draft. DC: coordinated the study. All authors contributed to the interpretation of data and revision of the manuscript draft and approved the final version. DC is the guarantor of this work.
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Martinovic, T., Vidicevic, S., Ciric, D. et al. The presence of Mott cells in the lymph nodes of rats with experimental autoimmune encephalomyelitis. Histochem Cell Biol 161, 287–295 (2024). https://doi.org/10.1007/s00418-023-02252-y
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DOI: https://doi.org/10.1007/s00418-023-02252-y