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Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds: successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats

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Abstract

Simon extracts are vitamin K1-rich food materials extracted from the leaves of the Simon sweet potato. Although vitamin K is known to stimulate bone formation, we postulated that Simon extracts also contain unknown biological compounds having the ability to regulate bone resorption. Here we prepared the vitamin K-free fraction from the Simon extracts and investigated the ability of this fraction on the differentiation of osteoclasts. A remarkable inhibitory effect of osteoclastogenesis was observed when osteoclast precursors were treated with this fraction in rat bone marrow culture systems as well as in a pure differentiation system using murine osteoclast precursor cell line. The vitamin K-free Simon extracts markedly suppressed severe bone destruction mediated by abundant osteoclasts associated with adjuvant-induced arthritis in rats. High performance liquid chromatography (HPLC) analysis revealed that the vitamin K-free Simon extracts contained three types of low molecular weight inhibitors for osteoclastogenesis; caffeic acid, chlorogenic acids and isochlorogenic acids. Among these substances, caffeic acid showed the most powerful inhibitory effects on osteoclastogenesis. Caffeic acid significantly suppressed expression of NFATc1, a key transcription factor for the induction of osteoclastogenesis. Our current study enlightened a high utility of the Simon extracts and their chemical components as effective regulators for bone resorption accompanied with inflammation and metabolic bone diseases.

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Acknowledgements

We thank Dr. Go Hirata of Department of Orthopedic Surgery, Faculty of Medical Science, Kyushu University for kind suggestions. This work was supported in part by a Grant for Scientific Research from the Japanese Ministry of Education, Science, and Culture (project 14571738). The authors have no conflicting financial interests.

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Authors and Affiliations

  1. Oral Cellular and Molecular Biology, Faculty of Dental Science, Kyushu University, Maidashi 3-1-1, Fukuoka, 812-8582, Japan

    Quan Yong Tang, Toshio Kukita, Ferry Sandra, Toshiyuki Watanabe, Kazuko Toh, Junpei Teramachi & Ichiko Miyamoto

  2. Kumamoto Flour Milling company, Hanazono 1-25-1, Kumamoto, 860-8625, Japan

    Yuki Ushijima, Yutaka Okuma & Sadamichi Kawasaki

  3. Pathology and Biodefense, Faculty of Medicine, Saga University, Nabeshima 5-1-1, Saga, 849-8501, Japan

    Akiko Kukita

  4. Oral Maxillofacial Oncology, Faculty of Dental Science, Kyushu University, Maidashi 3-1-1, Fukuoka, 812–8582, Japan

    Linda Rasubala

  5. Oral Anatomy and Cell Biology, Faculty of Dental Science, Kyushu University, Fukuoka, 812–8582, Japan

    Kengo Nagata, Zhou Wu & Tadahiko Iijima

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  1. Quan Yong Tang
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  2. Toshio Kukita
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Correspondence to Toshio Kukita.

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Quan Yong Tang is on leave from Hebei Medical University of China

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Tang, Q.Y., Kukita, T., Ushijima, Y. et al. Regulation of osteoclastogenesis by Simon extracts composed of caffeic acid and related compounds: successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats. Histochem Cell Biol 125, 215–225 (2006). https://doi.org/10.1007/s00418-005-0062-4

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