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The effect of subretinal viscoelastics on the porcine retinal function

  • Retinal Disorders
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Abstract

The functional consequence of long-term retinal detachment in the porcine model is examined by multifocal electroretinography (mfERG). Retinal detachment (RD) in humans leaves permanent visual impairment, despite anatomical successful reattachment surgery. To improve treatment, adjuvant pharmaceutical therapy is needed, and can only be tested in a suitable animal model. The porcine model is promising and the mfERG is well validated in this model. RD was induced in 18 pigs by vitrectomy and healon injection of various concentrations. Preoperatively and 6 weeks postoperatively eight animals were examined by mfERG. The major component P1 was analyzed statistically. Indirect ophthalmoscopy and bilateral color fundus photography (FP) were performed. Selected animals underwent high-resolution optical coherence tomography (OCT). Examination by ophthalmoscopy and FP showed that the RDs remained detached for the 6 weeks of follow-up. The P1 amplitude of the mfERG did not differ significantly between the detached areas, the surrounding attached areas, and the healthy eye (p = 0.25). Similarly, P1 implicit time did not differ between the areas (p = 0.85). The lack of functional consequences of long-term RD makes the porcine model unsuitable for examining adjuvant pharmaceutical RD treatment. Future studies should focus on foveated primates.

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Authors and Affiliations

  1. Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark

    Nina Fischer Sørensen, Rasmus Ejstrup, Thøger Frøsig Svahn, Birgit Sander, Jens Kiilgaard & Morten la Cour

  2. Department of Ophthalmology, Frederiksberg Hospital, University of Copenhagen, Copenhagen, Denmark

    Rasmus Ejstrup, Jens Kiilgaard & Morten la Cour

  3. Rigshospitalet, Department of Ophthalmology, University of Copenhagen, Copenhagen, Denmark

    Jens Kiilgaard

  4. Øjenafdelingen, Ndr. Ringvej 57, 2600, Glostrup, Denmark

    Nina Fischer Sørensen

Authors
  1. Nina Fischer Sørensen
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  2. Rasmus Ejstrup
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  3. Thøger Frøsig Svahn
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  4. Birgit Sander
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  5. Jens Kiilgaard
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  6. Morten la Cour
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Correspondence to Nina Fischer Sørensen.

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The Michaelsen foundation, The Danish Eye Health Society and The Velux Foundation is a non-profit foundations/organizations that support ophthalmological research.

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Sørensen, N.F., Ejstrup, R., Svahn, T.F. et al. The effect of subretinal viscoelastics on the porcine retinal function. Graefes Arch Clin Exp Ophthalmol 250, 79–86 (2012). https://doi.org/10.1007/s00417-011-1782-x

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  • DOI: https://doi.org/10.1007/s00417-011-1782-x

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