References
Binder S, Stanzel BV, Krebs I, Glittenberg C (2007) Transplantation of the RPE in AMD. Prog Retin Eye Res 26:516–554
Hu J, Bok D (2001) A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Mol Vis 7:14–19
Luo Y, Zhuo Y, Fukuhara M, Rizzolo LJ (2006) Effects of culture conditions on heterogeneity and the apical junctional complex of the ARPE-19 cell line. Invest Ophthalmol Vis Sci 47:3644–3655
Campochiaro PA, Hackett SF (1993) Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: implication of laminin and basic fibroblast growth factor as active components. Exp Eye Res 57:539–547
McKay BS, Burke JM (1994) Separation of phenotypically distinct subpopulations of cultured human retinal pigment epithelial cells. Exp Cell Res 213:85–92
Pfeffer BA (1991) Improved methodology for cell culture of human and monkey retinal pigment epithelium. Prog Retin Res 10:251–291
Peng S, Rahner C, Rizzolo LJ (2003) Apical and basal regulation of the permeability of the retinal pigment epithelium. Invest Ophthalmol Vis Sci 44:808–817
Chang CW, Ye L, Defoe DM, Caldwell RB (1997) Serum inhibits tight junction formation in cultured pigment epithelial cells. Invest Ophthalmol Vis Sci 38:1082–1093
von Recum HA, Okano T, Kim SW, Bernstein PS (1999) Maintenance of retinoid metabolism in human retinal pigment epithelium cell culture. Exp Eye Res 69:97–107
Villarroel M, Garcia-Ramirez M, Corraliza L, Hernandez C, Simo R (2009) Effects of high glucose concentration on the barrier function and the expression of tight junction proteins in human retinal pigment epithelial cells. Exp Eye Res 89:913–920
Rizzolo LJ, Chen X, Weitzman M, Sun R, Zhang H (2007) Analysis of the RPE transcriptome reveals dynamic changes during the development of the outer blood-retinal barrier. Mol Vis 13:1259–1273
Acknowledgements
This work was supported by an E. Schrödinger Fellowship grant (J2463- B13) from the Austrian Science Foundation to BVS, and with A. Rabensteiner Awards in 2005 and 2006 to BVS from the Austrian Ophthalmic Society. The authors wish to thank Jane Hu and Dr. Dean Bok, JSEI/UCLA for advice on culture media formulation and fetal RPE cell culture techniques. Miri Englander assisted with RPE culture maintenance. Dr. Itaya from the Zarbin laboratory is gratefully acknowledged for providing the silicone scraping spatula.
Author information
Authors and Affiliations
Corresponding author
Additional information
Disclaimer
Supported by E. Schrödinger postdoctoral fellowship grant from the Austrian Science Foundation (J2463-B13) to BVS, A. Rabensteiner Awards to BVS and an unrestricted research grant from the L. Boltzmann Institute to SB. Authors have full control over all primary data, and agree to allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review their data upon request.
Rights and permissions
About this article
Cite this article
Stanzel, B.V., Blumenkranz, M.S., Binder, S. et al. Longterm cultures of the aged human RPE do not maintain epithelial morphology and high transepithelial resistance. Graefes Arch Clin Exp Ophthalmol 250, 313–315 (2012). https://doi.org/10.1007/s00417-011-1624-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00417-011-1624-x