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Inhibition of experimental angiogenesis of cornea by somatostatin

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Abstract

Background.

This study evaluated the inhibitory activity of somatostatin 14 on the angiogenesis of cornea in vivo.

Methods.

Corneal neovascularization was induced with a pellet containing 90 ng of basic fibroblast growth factor (bFGF) in a rat corneal pocket model. Three kinds of pellets were made containing bFGF plus somatostatin (SST) 0 ng, 20 ng and 200 ng for the control group, group 1 and group 2, respectively. Neovascularization was observed biomicroscopically from day 4 to day 8, and the corneas were then examined for changes in histology. Quantitation of angiogenesis in the cornea was accomplished by caliper and image analysis.

Results.

The 200-ng dose of SST showed significant inhibition of both length and area of neovascularization on day 7 (0.62±0.11 mm vs 1.29±0.16 mm, 0.50±0.16 mm2 vs 1.35±0.29mm2, group 2 vs control; P <0.05). The 20 ng of somatostatin did not demonstrate any significant inhibition of neovascularization compared with the control group.

Conclusion.

Our study demonstrated that SST 14 can reduce bFGF-induced corneal angiogenesis. This shows the potential value of somatostatin in the treatment of corneal neovascularization.

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Fig. 1a–c.
Fig. 2a, b.
Fig. 3.

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Acknowledgements.

This work was supported by a project grant from Chang Gung Memorial Hospital Grand CMRP and National Science Council Grand NMRP. The authors thank Professor Nina Gloriani Barzaga for reading and editing the manuscript.

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Authors and Affiliations

  1. Department of Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan, ROC

    Pei-Chang Wu, Chi-Chang Liu, Chih-hsin Chen & Hsi-Kung Kou

  2. Department of Ophthalmology, Chang Gung Memorial Hospital, Taipei, Taiwan, ROC

    Su-Chin Shen

  3. Department of Pathology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan, ROC

    Ming-Tse Sung

  4. Gene Therapy Laboratory, Chang Gung Memorial Hospital, Kaohsiung, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien, 883, Taiwan, ROC

    Cheng-Yuan Lu, Wen-Ying Chou & Lin-Cheng Yang

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  1. Pei-Chang Wu
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Correspondence to Lin-Cheng Yang.

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The first, second, and third authors contributed equally

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Wu, PC., Liu, CC., Chen, Ch. et al. Inhibition of experimental angiogenesis of cornea by somatostatin. Graefe's Arch Clin Exp Ophthalmol 241, 63–69 (2003). https://doi.org/10.1007/s00417-002-0591-7

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  • DOI: https://doi.org/10.1007/s00417-002-0591-7

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