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Plasma levels of nitric oxide and stroke outcome

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Abstract

Production of reactive oxygen species after cerebral blood flow disruption may enhance tissue damage through multiple molecular pathways. Changes in nitric oxide (NO) metabolism and oxidative stress status were investigated in 47 patients with ischemic stroke by measuring plasma nitric oxide (NO) and peroxynitrite (ONOO–) levels.A correlation was sought between these two parameters and i) baseline stroke severity based on the National Institute of Health stroke scale (NIHSS) and ii) neurological outcome in terms of NIHSS changes from entry (T0) to 30 days after symptom onset (T1). The control group consisted of 30 age- and sex-matched healthy subjects. Mean plasma levels of ONOO– (arbitrary fluorescence number ± SD) were significantly higher in patients (7.70 ± 1.71 vs 5.35 ± 0.69, p < 0.001), whereas mean NO levels (nmol/mg protein) were significantly higher in controls (115.40 ± 12.40 vs. 51.10 ± 12.50, p < 0.001). Plasma ONOO– was significantly higher among patients with non-lacunar stroke (8.48 ± 1.50 vs. 6.95 ± 1.58 in those with lacunar stroke; p = 0.001), whereas NO levels were significantly higher among lacunar stroke patients (60.00 ± 7.86, vs. 41.77 ± 9.29 in patients with nonlacunar stroke; p < 0.001). Nitric oxide plasma levels were also associated with an unfavorable evolution in non-lacunar stroke, since a 10 unit increase in NO predicted a 1 point reduction in the NIHSS score at T1. Findings show that changes in NO metabolism may be considered as markers of brain injury in patients with ischemic stroke. Further work is needed to establish whether the amount of biochemical changes related to oxidative stress may influence outcome in these patients.

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Authors and Affiliations

  1. Clinica Neurologica, Università Politecnica delle Marche, Azienda Ospedaliero-Universitaria, Ospedali Riuniti, Via Conca 71, 60020, Ancona, Italy

    R. Taffi MD, M. Bartolini MD, L. Provinciali MD &  M. Silvestrini MD

  2. Institute of Biochemistry, Polytechnic University of Marche, Ancona, Italy

    L. Nanetti PhD, L. Mazzanti, A. Vignini PhD & F. Raffaelli PhD

  3. Center of Medical Statistics & Information, Technology, AFaR-Fatebenefratelli Hospital-Isola, Tiberina, Rome, Italy

    P. Pasqualetti PhD

  4. Neurological Clinic, Campus Biomedico University, Rome, Italy

    F. Vernieri MD

Authors
  1. R. Taffi MD
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  2. L. Nanetti PhD
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  3. L. Mazzanti
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  4. M. Bartolini MD
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  5. A. Vignini PhD
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  6. F. Raffaelli PhD
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  7. P. Pasqualetti PhD
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  8. F. Vernieri MD
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  9. L. Provinciali MD
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  10. M. Silvestrini MD
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Correspondence to M. Silvestrini MD.

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Taffi, R., Nanetti, L., Mazzanti, L. et al. Plasma levels of nitric oxide and stroke outcome. J Neurol 255, 94–98 (2008). https://doi.org/10.1007/s00415-007-0700-y

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  • DOI: https://doi.org/10.1007/s00415-007-0700-y

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