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Expression of oxygen-regulated protein 150 (ORP150) in skin wound healing and its application for wound age determination

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Abstract

Immunohistochemical study combined with morphometry was carried out to examine the expression of oxygen-regulated protein 150 (ORP150) using 58 human skin wounds of different ages (group I, 0–12 h; II, 1–5 days; III, 7–14 days; and IV, 17–21 days). In human wound specimens aged 4–12 h, neutrophils recruited at the wound showed no positive signals for ORP150. With the increase in wound age of ≥7 days, granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblasts with ORP150-positive reactions. In semi-quantitative analysis, the average of ORP150-positive ratios in group III was highest. In group III, all samples had an ORP150-positive ratio of >40%, and 17 samples showed >50%. In group IV, three out of ten samples showed a positive ratio of 40–45% and the remaining seven cases less than 40%. Collectively, with regard to the practical applicability with forensic safety, these observations suggest that an ORP150-positive ratio of >50% strongly indicates a wound age of 7–14 days.

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Acknowledgment

This study was financially supported by a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Science, Sports and Culture of Japan. We sincerely thank Ms. Mariko Kawaguchi for her excellent assistant in preparing this manuscript.

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Authors and Affiliations

  1. Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-8509, Japan

    Y. Ishida, A. Kimura & T. Kondo

  2. Department of Forensic and Social Environmental Medicine, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan

    T. Takayasu

  3. Institute for Legal Medicine, University of Munich, Frauenlobstrasse 7a, 80337, Munich, Germany

    W. Eisenmenger

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  1. Y. Ishida
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  2. A. Kimura
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  3. T. Takayasu
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  5. T. Kondo
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Correspondence to T. Kondo.

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Ishida, Y., Kimura, A., Takayasu, T. et al. Expression of oxygen-regulated protein 150 (ORP150) in skin wound healing and its application for wound age determination. Int J Legal Med 122, 409–414 (2008). https://doi.org/10.1007/s00414-008-0255-1

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  • DOI: https://doi.org/10.1007/s00414-008-0255-1

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