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Differential expression of cyclooxygenase-2 and cyclin D1 in salivary gland tumors

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Abstract

Purpose

Salivary gland tumors are complex and have a great histomorphological diversity; more than 30 histological subtypes are currently described and the study of proteins that help understand and differentiate these tumors is essential. We aimed to analyze the immunoexpression of cyclooxygenase-2 (COX-2) and cyclin D1 proteins in pleomorphic adenomas (PA), mucoepidermoid carcinomas (MEC) and adenoid cystic carcinomas (AdCC) of salivary glands.

Methods

A total of 38 PA, 12 AdCC and 12 MEC underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed semi-quantitatively. For statistical analysis, a significance level was set at p ≤ 0.05.

Results

Overall, these tumors were more prevalent in women (n = 37). The mean age of these patients was 58-year-old and the parotid gland was the most affected anatomic site (n = 33). All cases of AdCC and MEC showed immunopositivity to cyclin D1; however, 39.5% of the PAs were negative (p < 0.001). Regarding COX-2 immunoexpression, we observed that all cases of CME were positive, whereas 60.5% of the PA and 75% of the CAC analyzed were completely negative (p = 0.042).

Conclusions

The overexpression of COX-2, observed only in MEC, emphasizes that salivary gland tumors have different profiles. Cyclin D1 is more immunoexpressed in malignant tumors. Together, these immunohistochemical findings may be useful in differentiating the studied tumors.

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Acknowledgements

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES).

Funding

No funding was received.

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Authors and Affiliations

  1. Department of Oral Pathology and Special Care Patients, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227-Butantã, São Paulo, SP, Zip code: 05508-900, Brazil

    Jefferson da Rocha Tenório & Thalita Santana

  2. Oral Pathology, Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande Do Norte, Brazil

    Leorik Pereira da Silva

  3. Oral Pathology, School of Dentistry, University of Pernambuco, Camaragibe, PE, Brazil

    Marília Gabriela de Aguiar Xavier & Ana Paula Veras Sobral

  4. Biological Sciences Academic Center, School of Dentistry, Federal University of Campina Grande-UFCG, Patos, Paraíba, Brazil

    George João Ferreira do Nascimento

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Correspondence to Jefferson da Rocha Tenório.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ethical approval for this study was obtained from the relevant local ethics committees (CEP/UPE no 243/11).

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Tenório, J.d.R., da Silva, L.P., Xavier, M.G.d.A. et al. Differential expression of cyclooxygenase-2 and cyclin D1 in salivary gland tumors. Eur Arch Otorhinolaryngol 275, 2341–2347 (2018). https://doi.org/10.1007/s00405-018-5058-5

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