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Early effects of irradiation on laryngeal mucosa in a gastroesophageal reflux model: an experimental study

  • Head and Neck
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Abstract

Objective

The aim of this study was to evaluate the early histopathological changes of gastroesophageal reflux and irradiation on laryngeal mucosa in rats.

Study design

Animal study.

Setting

Experimental animal laboratory, tertiary referral center.

Subject and method

Twenty-four adult female Wistar Albino rats were grouped as: control (n = 6), reflux and irradiation (n = 10), and irradiation (n = 8). Rats were operated to create a reflux model 30 days before irradiation. Ionizing radiation was administered in a single fraction of a 20 Gy to the larynx. Laryngeal tissue samples were taken at the 4th day of irradiation and all specimens underwent histopathological examination.

Results

Edema and vascular dilation in lamina propria were higher in the reflux and irradiation, and irradiation groups than control group. Inflammation was higher in the reflux and irradiation group than the control group. Inflammation in squamous epithelium was higher in the reflux and irradiation and irradiation groups compared to the control group. Inflammation in the squamous epithelium of the irradiation group was higher than the reflux and irradiation group. In the respiratory tract epithelium, inflammation was higher in the reflux and irradiation group; additionally, a significant loss of cilia was present in the reflux and irradiation and irradiation groups while pseudostratification was higher in the reflux and irradiation group.

Conclusion

Ionizing radiation-induced inflammation may increase on previously inflammated area due to gastroesophageal reflux. Therefore, it may be helpful to investigate and treat the reflux in laryngeal cancer patients that will receive ionizing radiation.

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Funding

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Authors and Affiliations

  1. Ear Nose and Throat Clinic, Health Sciences University Fatih Sultan Mehmet Training and Research Hospital, Istanbul, Turkey

    Süleyman Oyan, Arzu Tatlıpınar & Serhan Keskin

  2. Department of Radiation Oncology, Marmara University School of Medicine, Istanbul, Turkey

    Beste M. Atasoy

  3. Pathology Clinic, Health Sciences University Haydarpaşa Numune Training and Research Hospital, Istanbul, Turkey

    Pembegül Güneş

  4. Experimental Animal Laboratory, Marmara University School of Medicine, Istanbul, Turkey

    Dilek Özbeyli

  5. Clinic of Radiation Oncology, Ministry of Health-Marmara University Pendik Training and Research Hospital, Istanbul, Turkey

    Ayse Dağlı Değerli

Authors
  1. Süleyman Oyan
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  2. Arzu Tatlıpınar
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  3. Beste M. Atasoy
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  4. Pembegül Güneş
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  5. Dilek Özbeyli
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  6. Serhan Keskin
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  7. Ayse Dağlı Değerli
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Corresponding author

Correspondence to Arzu Tatlıpınar.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Research involving human participants and animal

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Oyan, S., Tatlıpınar, A., Atasoy, B.M. et al. Early effects of irradiation on laryngeal mucosa in a gastroesophageal reflux model: an experimental study. Eur Arch Otorhinolaryngol 275, 2089–2094 (2018). https://doi.org/10.1007/s00405-018-5010-8

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  • DOI: https://doi.org/10.1007/s00405-018-5010-8

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