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Nuclear factor kappa B pathway down-regulates aquaporin 5 in the nasal mucosa of rats with allergic rhinitis

  • Rhinology
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Abstract

Nuclear factor kappa B (NF-κB) induces gene transcription by binding CREB-binding protein (CBP). The aim of the study was to detect the mechanisms by which NF-κB pathway regulated aquaporin 5 (AQP5) in the nasal mucosa of rats with allergic rhinitis (AR). Rats were divided into control (group C), model (group M), low-dose proline dithiocarbamate (PDTC) (group L) and high-dose PDTC (group H) groups. AR model was established by the sensitization with ovalbumin, then groups L and H were treated with PDTC (50 or 100 mg/kg/day) for 5 days. AQP5, interleukin-1β, NF-κBp65 and phosphorylated cAMP-response element binding protein (p-CREB) were detected by immunohistochemistry, Western blotting or real-time PCR. AQP5 expression in group M was lower than in group C, but in groups L and H it increased. NF-κBp65 expression in group M was higher than group C, but in groups L and H it reduced. p-CREB expression in group M was lower than group C, but in groups L and H it increased. Interleukin-1β gene level in group M was higher than group C, but in groups L and H it was lower. These data show that the NF-κB pathway could down-regulate AQP5 by interleukin-1β which inhibited CREB phosphorylation or by NF-κBp65 which competitively bound CBP.

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Acknowledgments

This study was carried out in the neurobiological laboratory of Fujian Medical University, so we warmly thank Prof. Weiwang, the director of the neurobiological Laboratory. This study was approved by Fujian Medical University on Principles of Laboratory Animal Care and was performed in accordance with the German Law on protection of Animals.

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The authors do not have any possible conflicts of interest.

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Authors and Affiliations

  1. Fujian Medical University, No. 88, Jiaotong Road, Fuzhou, 350004, Fujian, China

    Weiwei Wang & Ming Zheng

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  1. Weiwei Wang
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  2. Ming Zheng
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Correspondence to Ming Zheng.

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Wang, W., Zheng, M. Nuclear factor kappa B pathway down-regulates aquaporin 5 in the nasal mucosa of rats with allergic rhinitis. Eur Arch Otorhinolaryngol 268, 73–81 (2011). https://doi.org/10.1007/s00405-010-1282-3

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  • DOI: https://doi.org/10.1007/s00405-010-1282-3

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